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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,397 results for: bli

Showing: 5941 - 5960 of 9397 results

  1. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

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  2. p2B-55

    Plasmid
    #177924
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    TDH3
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177924-plasmid-map-sequence-id-349994

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  3. 3CEG

    Plasmid
    #60107
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    BIRC6:SDC136-F10:C41228 (BIRC6 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. HA Display H3_A/Hong Kong/1/1968

    Plasmid
    #206506
    Purpose
    Expresses H3_Hong Kong/1/1968 in the surface of mammalian cells
    Depositor
    IIya Finkelstein
    Insert
    H3_A/Hong Kong/1/1968
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    deleted amino acids 1651 to 1735
    Promoter
    CMV
    Available Since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    206506-plasmid-map-sequence-id-408102

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  5. OptoEphB2KD-mCherry

    Plasmid
    #92044
    Purpose
    Expresses kinase-dead mutant of OptoEphB2 labeled with mCherry
    Depositor
    Ji Yu
    Insert
    EPHB2 (EPHB2, CRY2 Mustard Weed, Human)
    Tags
    mCherry
    Expression
    Mammalian
    Available Since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    92044-plasmid-map-sequence-id-182373

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  6. pcDNA3.1+ eiF4A2 myc HIS

    Plasmid
    #71658
    Purpose
    mammalian expression of eiF4A2
    Depositor
    Gideon Dreyfuss
    Insert
    EIF4A2 (EIF4A2 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. pET28-6His-SUMO-Nsp10

    Plasmid
    #154959
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154959-plasmid-map-sequence-id-307848

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  8. Hs.HRAS

    Plasmid
    #83181
    Purpose
    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Available Since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83181-plasmid-map-sequence-id-158144

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  9. GLP-1R-SmBiT Fusion Vector

    Plasmid
    #236845
    Purpose
    Express GLP-1R-SmBiT in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    GLP-1R (GLP1R Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236845-plasmid-map-sequence-id-471845

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  10. LgBiT-PIK3CA Fusion Vector

    Plasmid
    #237092
    Purpose
    Express LgBiT-PIK3CA Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    PIK3CA (PIK3CA Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237092-plasmid-map-sequence-id-473321

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  11. PIK3CA-SmBiT Fusion Vector

    Plasmid
    #237093
    Purpose
    Express PIK3CA-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    PIK3CA (PIK3CA Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237093-plasmid-map-sequence-id-473322

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  12. PIK3CA-LgBiT Fusion Vector

    Plasmid
    #237094
    Purpose
    Express PIK3CA-LgBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    PIK3CA (PIK3CA Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237094-plasmid-map-sequence-id-473323

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  13. KAT2B

    Plasmid
    #39002
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KAT2B (KAT2B Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pLV-HuGDNF

    Plasmid
    #182946
    Purpose
    To expres Human GDNF with optimized codon usage for better expression
    Depositor
    Pantelis Tsoulfas
    Insert
    Human GDNF (GDNF Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV enhancer
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182946-plasmid-map-sequence-id-360108

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  17. Aiolos AA130-189 in Artichoke

    Plasmid
    #74451
    Purpose
    Monitor post-translational degradation of Aiolos AA130-189 (Lentiviral, SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Insert
    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Promoter
    SFFV and EF1a
    Available Since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    74451-plasmid-map-sequence-id-140674

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  18. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid
    #52054
    Purpose
    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)
    Depositor
    Jesse Rinehart
    Inserts
    SepRS
    EF-Sep
    5x tRNA Sep
    Expression
    Bacterial
    Mutation
    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…
    Promoter
    Ptac
    Available Since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    52054-plasmid-map-sequence-id-438919

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  19. AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA

    Plasmid
    #51094
    Purpose
    Cre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    oChIEF(E163A/T199C)
    Use
    AAV
    Tags
    P2A-dTomato
    Expression
    Mammalian
    Mutation
    E163A/T199C
    Promoter
    EF1a
    Available Since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51094-plasmid-map-sequence-id-79315

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  20. ECHS1

    Plasmid
    #38845
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ECHS1 (Echs1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 5941 - 5960 of 9397 results