We narrowed to 7,668 results for: CCH

1. pETM6-G6-vioABECD

   Plasmid

   #66536

   Purpose

   pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Inserts

   VioA
   VioB
   VioE
   VioC
   VioD
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Mutant T7 Promoter - G6

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. M4786 KanMX::hisG-URA3-HisG Disruptor Converter

   Plasmid

   #51690

   Purpose

   Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   hisG-URA3-hisG (URA3 Budding Yeast)
   

   Use

   Yeast marker swap

   Expression

   Yeast

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYSD099

   Plasmid

   #212923

   Purpose

   A MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ABKAR

   Plasmid

   #140051

   Purpose

   Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

   Depositor

   Jin Zhang

   Article

   Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

   Insert

   ABKAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDuRCC-N

   Plasmid

   #81194

   Purpose

   Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please view depositor comments below and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

   Plasmid

   #167898

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTpal-fdc

   Plasmid

   #78288

   Purpose

   Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

   Depositor

   David Nielsen

   Article

   McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

   Inserts

   PAL2 (PAL2 Mustard Weed)
   FDC1 (FDC1 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   *see below

   Promoter

   trc

   Available Since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-ATG8(414)/GFP-AUT7(414)

   Plasmid

   #49424

   Purpose

   Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

   Depositor

   Daniel Klionsky

   Article

   Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

   Insert

   autophagy-related 8 (ATG8 Budding Yeast)
   

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Promoter

   ATG8

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. -227 hTF

   Plasmid

   #15442

   Depositor

   Nigel Mackman

   Article

   Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

   Insert

   Tissue factor promoter (F3 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   July 27, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pILGFPE9

    Plasmid

    #83545

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

    Plasmid

    #79383

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Zhang 2.0 + scaffold
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSIIbsr-6013x

    Plasmid

    #108654

    Purpose

    An HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    Raichu-EV
    

    Use

    Lentiviral

    Tags

    XFP

    Expression

    Mammalian

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB2794

    Plasmid

    #63651

    Purpose

    pCfB2794 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    TEF1 promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCOLA-Spec-yibDp-Erv1p

    Plasmid

    #212094

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    Sulfydryl oxidase (ERV1 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCPP5238

    Plasmid

    #128711

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopG1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. NAX_CAGGS_CAS9_m4_VPR_mcherry

    Plasmid

    #167886

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-AktAR-Kras

    Plasmid

    #123357

    Purpose

    FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

    Depositor

    Jin Zhang

    Article

    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

    Insert

    AktAR-Kras
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG306-GPD-VMA1 chr I

    Plasmid

    #41896

    Purpose

    Yeast expression of VMA1.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    VMA1 (VMA1 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Contains silent mutation at 342A-G and L87S mutat…

    Promoter

    GPD

    Available Since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCPP5108

    Plasmid

    #128726

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopAA1-2
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCPP5107

    Plasmid

    #128725

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopAA1-1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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