We narrowed to 6,763 results for: poly
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Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
TagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I26 pUBQ10 GG0-GG1
Plasmid#195902PurposeLevel 0 pUBQ10 promoter with GG0 and GG1 golden gate linkersDepositorInsertpUBQ10 (UBQ10 Mustard Weed)
UseSynthetic BiologyAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVH1001-Tier2(ColE1 ori)
Plasmid#169599PurposeTier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and ColE1 ori).DepositorTypeEmpty backboneExpressionMammalianAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22MS1-2xStrep
Plasmid#170314PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22MS1
TagsStrep-Tag IIExpressionInsectPromoterPolyhedrinAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc393
Plasmid#161953PurposeDemonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmidDepositorInsertpSB4A3-T-PT7-B30-mScarlet-I-3T
ExpressionBacterialPromoterPT7Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Myo 9 Avi+Flag
Plasmid#134969PurposeExpression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1DepositorAvailable SinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-HypaCAS9-pA
Plasmid#131467PurposeExpresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized HypaCas9
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGS-BacA-21222-FLP-3xFLAG
Plasmid#109165PurposeEncodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInsertFlp
Tags3xFLAGExpressionInsectPromoterPolyhedrinAvailable SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBac(3xP3-gTc’v; Valcyrie.LR)
Plasmid#119066PurposeValcyrie construct for CRISPR-mediated lox excision in Tribolium castaneum (high)DepositorInsertspBacL
Tc-U6a-Lox.L
Tc-hsp68 Promoter
NLS-Cas9
Tc-hsp68 3'UTR
Tc-U6B-Lox.R
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR
ExpressionInsectAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-MMP
Plasmid#112632PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-MMP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3R112C/T180C-GG
Plasmid#85093PurposeHost vector to fuse a gene at the C-terminus of PCNA3 variant (R112C/T180C)DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationchanged Arginine 112 to Cysteine and Threonine 18…PromoterT7 promoterAvailable SinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
p1G108C-GG
Plasmid#85092PurposeHost vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)DepositorInsertDNA polymerase sliding clamp 1
TagsHis6 tagExpressionBacterialMutationchanged Glycine 108 to CysteinePromoterT7 promoterAvailable SinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 T180C
Plasmid#66181PurposeExpresses the T180C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationT180CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 R112C
Plasmid#66180PurposeExpresses the R112C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationR112CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 E105C
Plasmid#66176PurposeExpresses the E105C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
TagsHis6 tagExpressionBacterialMutationE105CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HISX6-TEV-RSP4
Plasmid#176693PurposeExpression of Chlamydomonas reinhardtii RSP4 recombinant protein in insect cellsDepositorInsertRSP4
TagsHISX6ExpressionInsectMutationNonePromoterpolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pLIB-RSP5
Plasmid#176694PurposeExpression of Chlamydomonas reinhardtii RSP5 recombinant protein in insect cellsDepositorAvailabilityAcademic Institutions and Nonprofits only -
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pFS462
Plasmid#89065Purposehis7 integration plasmid with Z3EVpr:GFPDepositorInsertZ3EV promoter GFP-NLS leu1 C-terminal 300 nt
UseS. pombe his7 integration vectorPromoterZ3EVAvailable SinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-amidase
Plasmid#128135PurposeThe cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertamidase
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only