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pME-V5-mClover-KRAS4A-WT (JDW 812)

Plasmid

#242566

Purpose

Gateway middle entry clone containing an mClover3 fused human KRAS4A WT.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

KRAS4A (WT) (KRAS Human)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-NES-SomaFRCaMPi

Plasmid

#232836

Purpose

AAV transfer plasmid for CAG-mediated Cre-dependent expression of soma-targeted FRCaMPi

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-FRCaMPi (NES Synthetic)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-NES-SomaKGECO1

Plasmid

#232839

Purpose

AAV transfer plasmid for Syn-mediated expression of soma-targeted KGECO1

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-SomaKGECO1 (NES Synthetic)

Use

AAV

Expression

Mammalian

Promoter

Syn

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NES-FRCaMPi-P2A-mGreenLantern

Plasmid

#232841

Purpose

AAV transfer plasmid for CAG-mediated co-expression of FRCaMPi and mGreenLantern

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-FRCaMPi (NES Synthetic)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NES-KGECO1-P2A-mGreenLantern

Plasmid

#232843

Purpose

AAV transfer plasmid for CAG-mediated co-expression of KGECO1 and mGreenLantern

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Inserts

NES-KGECO1 (NES Synthetic)
mGreenLantern

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NES-jRGECO1-P2A-mGreenLantern

Plasmid

#232844

Purpose

AAV transfer plasmid for CAG-mediated co-expression of jRGECO1 and mGreenLantern

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Inserts

NES-jRGECO1 (NES Synthetic)
mGreenLantern

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE

Plasmid

#224936

Purpose

pAAV vector for flippase(FLP)-dependent OCaMP (orange calcium indicator) expression under the control of EF1a promoter

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

Ef1a

Available Since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-Syn-NES-SomajRGECO1a

Plasmid

#232842

Purpose

AAV transfer plasmid for Syn-mediated expression of soma-targeted jRGECO1a

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-SomajRGECO1a (NES Synthetic)

Use

AAV

Expression

Mammalian

Promoter

Syn

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NONO-TFE3

Plasmid

#237641

Purpose

For overexpression of mEGFP-NONO-TFE3

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NONO-TFE3 (NONO, TFE3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-YAP-MAMLD1-KS

Plasmid

#237676

Purpose

For overexpression of mEGFP-YAP-MAMLD1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

YAP-MAMLD1-KS (YAP1, MAMLD1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NONO-TFE3-KS

Plasmid

#237681

Purpose

For overexpression of mEGFP-NONO-TFE3-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NONO-TFE3-KS (NONO, TFE3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NUP98-DDX10-Mut1

Plasmid

#237647

Purpose

For overexpression of mEGFP-NUP98-DDX10-Mut1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NUP98-DDX10-Mut1 (NUP98, DDX10 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NUP98-DDX10-Mut2

Plasmid

#237648

Purpose

For overexpression of mEGFP-NUP98-DDX10-Mut2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NUP98-DDX10-Mut2 (NUP98, DDX10 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NUP98-DDX10-KS-FtoG

Plasmid

#237645

Purpose

For overexpression of mEGFP-NUP98-DDX10-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NUP98-DDX10-KS-FtoG (NUP98, DDX10 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-FUS-gRNA

Plasmid

#237685

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to FUS locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

FUS gRNA (FUS Human)

Use

CRISPR

Tags

mCherry

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJL-mGold2s

Plasmid

#231761

Purpose

Expression of mGold2s in yeast cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s

Expression

Yeast

Mutation

mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

pTDH(GAP promoter)

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-CAG_G-PTEN

Plasmid

#227438

Purpose

An AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner.

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN sensor (Pten Rat)

Use

AAV

Tags

CD-sREACh and mEGFP

Expression

Mammalian

Mutation

R14G

Promoter

pCAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pJL-mGold2t

Plasmid

#231762

Purpose

Expression of mGold2t in yeast cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t

Expression

Yeast

Mutation

mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

pTDH(GAP promoter)

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

Plasmid

#229816

Purpose

A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-EGFP-stop-lox

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

Plasmid

#229826

Purpose

Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet-I

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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