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LC201: pMVP/PB/Puro-DEST

Plasmid

#121874

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HOXB2 (human) HIS-tag pET

Plasmid

#8522

Depositor

Corey Largman

Publication

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB2 (HOXB2 Human)

Tags

His and T7

Expression

Bacterial

Mutation

Starts at AA #2; makes protein by Western

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3. Flag-CSL WT

Plasmid

#167004

Purpose

Mammalian expression of CSL WT

Depositor

Gian-Paolo Dotto

Publication

Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

Insert

CSL (RBPJ Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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mTiam1-mcherry-sspB in pcDNA3.1

Plasmid

#85221

Purpose

Role of Rac selective GEF domain from Tiam1 in immune cell migration

Depositor

Narasimhan Gautam

Publication

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Inserts

mTiam1 (1030-1406) (Tiam1 Mouse)
mcherry
sspB

Tags

none

Expression

Mammalian

Promoter

CMV

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMito-iRFP713

Plasmid

#45465

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP713

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pmyc-GFP-TNRC6A-NES-mut

Plasmid

#42001

Depositor

Kumiko Ui-Tei

Publication

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

F951A, F955A, I958A, F960A

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 merlin 308-595

Plasmid

#11628

Depositor

Vijaya Ramesh

Publication

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Carboxy-terminal half of merlin isoform 1.

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
mCherry-DRP1 K38A

Plasmid

#232350

Purpose

Expresses K38A variant of human DRP1 with mCherry tag

Depositor

Samantha Lewis

Publication

Begeman et al Sci Adv. 2025 Apr 18;11(16):eads6830. do

Insert

DRP1 (DNM1L Human)

Tags

mCherry

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDEST-mCherry-ARHGEF2

Plasmid

#207959

Purpose

Expression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry tag

Depositor

Johanna Ivaska

Publication

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

ARHGEF2 (ARHGEF2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Integrin Beta1-mPA-GFP

Plasmid

#186302

Purpose

Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

Depositor

Pakorn Kanchanawong

Publication

Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

Insert

Integrin Beta 1 (ITGB1 Human)

Tags

mPA-GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HER3-DMVF-181-R

Plasmid

#87621

Purpose

C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

HER3-DMVF181-C (ERBB3 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ash-mCerulean

Plasmid

#69579

Purpose

Expresses p53 tagged with mCerulean

Depositor

Galit Lahav

Publication

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-C1-FLAG-GFP-Ubl4A

Plasmid

#86921

Purpose

To express Ubl4A tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Publication

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pINDgw Cbx1 V5 EcoDam

Plasmid

#59203

Purpose

Mammalian DamID positive control plasmid. Expresses Cbx1-V5-Dam.

Depositor

Bas van Steensel

Publication

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Insert

Cbx1 (Cbx1 Mouse)

Use

Damid

Tags

Dam and V5

Expression

Mammalian

Promoter

heat shock minimal pomoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-T1977R

Plasmid

#69011

Purpose

activating MTOR mutation

Depositor

David Sabatini

Publication

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-T1977R (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Threonine 1977 to Arginine

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCDNA3.1-hMcl-1(S159A)

Plasmid

#25374

Depositor

Roger Davis

Publication

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1(S159A) (MCL1 Human)

Tags

V5 and His tag

Expression

Mammalian

Mutation

changed serine 159 to alanine

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MAP4K3-GFP K45E

Plasmid

#21734

Depositor

L. Miguel Martins

Publication

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Mutation of the ATP-binding site (K45E). This ver…

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA 3.3 EGFP

Plasmid

#115786

Purpose

Expresses EGFP in mammalian cells

Depositor

Harald Junge

Publication

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB401

Plasmid

#68666

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pbacNeoR

Plasmid

#26349

Depositor

Ben Lehner

Publication

Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

Insert

Bacterial promoter::NeoR::bacterial 3p flank

Expression

Bacterial

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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