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pTBX1-αMoonTag-Tn5

Plasmid

#254522

Purpose

For use with CoCUT&Tag method, binds MoonTag epitope to tether Tn5 transposase

Depositor

Derek Janssens

Article

Sun et al bioRxiv 2026.04.27.721191

Insert

anti-MoonTag::Tn5

Tags

Chitin Binding Domain and Mxe GyrA intein

Expression

Bacterial

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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pTY1-LacI

Plasmid

#164831

Purpose

LacI protein regulates the expression of a tetracycline resistance gene and yellow fluorescent protein

Depositor

Drew Tack

Article

Tack et al Mol Syst Biol. 2021 Mar;17(3):e10179. do

Insert

LacI

Use

Synthetic Biology

Promoter

Ptac

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pESC-URA (V5 and HA)

Plasmid

#222485

Purpose

Co-expression of two genes in yeast with V5 and HA tags and URA3 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-TRP (V5 and HA)

Plasmid

#222488

Purpose

Co-expression of two genes in yeast with V5 and HA tags and TRP1 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP8

Plasmid

#22608

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP8 (USP8 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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pMA7CR_2.0

Plasmid

#73950

Purpose

pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

Depositor

Alex Nielsen

Article

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Inserts

cas9
lambda beta
dam
recX

Expression

Bacterial

Promoter

pAra and pTet

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pJP_Rep00

Plasmid

#219677

Purpose

Light-inducible repressilator (modified from pLPT107). PT3lacO promoter controls the expression of TetR node.

Depositor

Yolanda Schaerli

Article

Park et al Nat Commun. 2024 Aug 23;15(1):7284. doi:

Insert

PT3lacO

Use

Synthetic Biology

Promoter

PT3lacO

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSHARK2

Plasmid

#248168

Purpose

pBBR1 plasmid with constituitive lambda-CI (J23101-Bujard-Lambda-CI) and PirWT (PTet-B0033-PirWT) expression

Depositor

Thomas Gorochowski

Article

Hina-Nilesh Joshi et al ACS Synth Biol. 2026 Jan 13. doi: 10.102

Insert

LambdaCI-PirWT

Expression

Bacterial

Mutation

None

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D NAPA-A SV40

Plasmid

#92281

Purpose

FRET probe; extracellular membrane tethered eGFP

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Astrocyte

Use

AAV

Promoter

GfaABC1D

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pESC-LEU (V5 and HA)

Plasmid

#222486

Purpose

Co-expression of two genes in yeast with V5 and HA tags and LEU2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTcINDEX-GW

Plasmid

#234713

Purpose

Used to express any CDS in T. cruzi in a tetracycline regulatable manner. Modified from Taylor et al. (2006) for easy cloning using Gateway recombination cassette.

Depositor

Esteban Serra

Article

Alonso et al Mem Inst Oswaldo Cruz. 2014 Dec;109(8):1

Type

Empty backbone

Use

Trypanosomatid expression

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Expression

Bacterial

Promoter

Pdhfr

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-TTP(C147R)-GG-PUF

Plasmid

#52138

Purpose

Receiving vector for PUM-HD assembly and expression in mammalian cells

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Type

Empty backbone

Tags

(GGGGS)3GGGG linker, 3xFlag, PUM-HD flanking regi…

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ134B

Plasmid

#179216

Purpose

Golden Gate entry vector; 4th gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Promoter

AtU3

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-UTY-F

Plasmid

#17439

Purpose

Expression of UTY in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

UTY (UTY Human)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-CD151-7

Plasmid

#57125

Purpose

Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

CD151 (CD151 Human)

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Flag-HA-USPL1

Plasmid

#22607

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USPL1 (USPL1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pQFT-mNG-Pcon-Scar

Plasmid

#203678

Purpose

pQFT-mNG with constitutively expressed mScarlet-I

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Inserts

mNeonGreen
mScarlet-I

Expression

Bacterial

Promoter

PQJ and Phyb18

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-pgaC

Plasmid

#154919

Purpose

aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting pgaC

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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