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Plasmid#133072PurposeEntry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.DepositorTypeEmpty backboneUseEntry vectorTags3C-Flag-TwinStrep and TwinStrep-Flag-3CExpressionMammalianMutationPromoterCMVT (Enhanced CMV)Available sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only
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pET15b-eGO-Matryoshka-LE-LP
Plasmid#100017PurposeBacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpEGFP-LSSmOrange
UseTags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s-T78H
Plasmid#100023PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutationDepositorInsertsfGCaMP6s-T78H
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Claudin7-C-12
Plasmid#54041PurposeLocalization: Tight Junctions, Excitation: 487, Emission: 509DepositorInsertClaudin7 (CLDN7 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-Claudin7-N-12
Plasmid#54042PurposeLocalization: Tight Junctions, Excitation: 487, Emission: 509DepositorInsertClaudin7 (CLDN7 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
ElectricPk
Plasmid#40314DepositorInsertElectricPk
UseTagscpEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTol-Promoter:NLS-tdMCP-eGFP
Plasmid#86248PurposeFor generating transgenic lines that ubiquitously expressing the fluorescent MCP reporterDepositorInsertbeta Actin promoter:NLS-tdMCPeGFP
UseUsed with transposase to make transient or stable…TagsNLS-tdMCP-eGFPExpressionMutationPromoterBeta ActinAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AMPKAR
Plasmid#192446PurposeGenetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.DepositorInsertExRai-AMPKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mito-ExRai-AMPKAR
Plasmid#192448PurposeExRai-AMPKAR targeted to the mitochondrial outer membrane.DepositorInsertMito-ExRai-AMPKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-ExRai-AMPKAR
Plasmid#192449PurposeExRai-AMPKAR targeted to the lysosomal membrane.DepositorInsertLyso-ExRai-AMPKAR
UseTagsFull-length LAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only