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We narrowed to 126 results for: nuclear bfp

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Showing: 61 - 90 of 126 results

  4. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Article
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and hPGK
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  5. pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid
    #87829
    Purpose
    High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    MTS
    NLS
    PTS1
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87829-plasmid-map-sequence-id-175007

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  8. pSLQ12774-PHR-EF1a-leucine zipper-GFP11-NES-2A-CD19 (no ICD)-P2A-puroR-2A-BFP-NLS

    Plasmid
    #252642
    Purpose
    Human expression vector containing EF1a promoter, GFP11 along with a leucine zipper, CD19 (no intracellular domain), nuclear BFP, and puromycin resistance gene
    Depositor
    Stanley Qi
    Article
    Chen et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.
    Insert
    GFP11-leucine-zipper, CD19 (no ICD), PuroR, BFP-NLS
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    March 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    252642-plasmid-map-sequence-id-504281

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  9. H2B-Electra2

    Plasmid
    #218929
    Purpose
    Electra2 fused to human H2B protein for nuclear localization
    Depositor
    Amro Hamdoun
    Insert
    H2B-Electra2 (H2B Synthetic, Human, Entacmaea quadricolor)
    Use
    Synthetic Biology
    Tags
    Electra2 and H2B
    Expression
    Mammalian
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218929-plasmid-map-sequence-id-433882

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  10. PiggyBac-APEX-V5-NES

    Plasmid
    #229082
    Purpose
    Doxycycline-inducible expression of nuclear export signal (NES) fused to V5-tag and APEX for proximity labeling, containing BFP in the backbone
    Depositor
    Eran Hornstein
    Insert
    NES (TARDBP Human)
    Tags
    APEX (D11G mutation), BFP, and V5
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    229082-plasmid-map-sequence-id-462274

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  13. pJH127

    Plasmid
    #162483
    Purpose
    SEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162483-plasmid-map-sequence-id-320838

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  14. pJH130

    Plasmid
    #162484
    Purpose
    SEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162484-plasmid-map-sequence-id-320834

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  15. pJH126

    Plasmid
    #162482
    Purpose
    SEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162482-plasmid-map-sequence-id-320829

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  16. pJPF0117

    Plasmid
    #130267
    Purpose
    Lentiviral vector for the co-expression of cytoplasmic bPAC and nuclear tagBFP
    Depositor
    Hana El-Samad
    Article
    Fonseca et al bioRxiv 2020, 747030
    Insert
    bPAC
    Use
    Lentiviral
    Tags
    tagBFP
    Expression
    Mammalian
    Promoter
    hPGK
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    130267-plasmid-map-sequence-id-254858

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  19. pMTB-Multibow-hB

    Plasmid
    #60992
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear EBFP2.
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    EBFP2
    Use
    Zebrafish
    Tags
    histone 2B tag
    Promoter
    Bactin2
    Available Since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. JPF0466

    Plasmid
    #124037
    Purpose
    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a tagBFP-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    pHR_pEF1a-iRFP713_tagBFP sgRNA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124037-plasmid-map-sequence-id-262668

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  25. JPF0468

    Plasmid
    #124038
    Purpose
    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    pHR_pEF1a-iRFP713_mScarletsgRNA_tagBFP sgRNA
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124038-plasmid-map-sequence-id-252546

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  26. Tol2-CAG::Nucbow

    Plasmid
    #158992
    Purpose
    Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Article
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Nucbow
    Tags
    All FPs are fused to H2B
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158992-plasmid-map-sequence-id-311797

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  27. PB-CAG::Nucbow

    Plasmid
    #158996
    Purpose
    Multicolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Article
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Nucbow
    Tags
    All FPs are fused to H2B
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158996-plasmid-map-sequence-id-311805

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Showing: 61 - 90 of 126 results