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Plasmid#85224PurposeshLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.DepositorInsertFirefly Luciferase
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shB3GNT5.1
Plasmid#110325PurposeTRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2DepositorInsertB3GNT5 (B3GNT5 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shGLS
Plasmid#110319PurposeshGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene and express monomeric Kusabira-Orange2DepositorInsertGLS Glutaminase (GLS Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast shLuc
Plasmid#110409PurposeshLuc (Target TTACGCTGAGTACTTCGA), silence LUC gene, blasticidin selection.DepositorInsertLuciferase
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA PolIII)Available sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-AP-1-mCherry
Plasmid#118095PurposeFluorescent reporter for AP-1 activationDepositorInsertmCherry
UseRetroviralTagsExpressionMutationPromoterminimal promoter with AP-1 responsive elementAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast shGAC
Plasmid#110410PurposeshGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.DepositorInsertGLS glutaminase (GLS Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA Pol III)Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shGBP1.1.mKO2
Plasmid#85209PurposeTRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.DepositorInsertGBP1 (guanylate binding protein 1) (GBP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shGBP1.2.mKO2
Plasmid#85210PurposeTRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.DepositorInsertGBP1 (guanylate binding protein 1) (GBP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shHRASLS.2
Plasmid#110324PurposeTRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2DepositorInsertHRASLS (PLAAT1 Homo sapiens, Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_EIF3I:1-325
Plasmid#211280PurposeMammalian expression of full-length human EIF3I. C-terminal HiBiT tag.DepositorInsertEIF3I:M1-A325 (EIF3I Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_EIF3I:1-325
Plasmid#211206PurposeMammalian expression of full-length human EIF3I. N-terminal HiBiT tag.DepositorInsertEIF3I:M1-A325 (EIF3I Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_EIF3I:1-325
Plasmid#210910PurposeInsect expression of full-length human EIF3I. N-terminal 6X His tag with TEV cleavage.DepositorInsertEIF3I:M1-A325 (EIF3I Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS Coupler 1
Plasmid#185824PurposeCloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].DepositorInsert(GlyGlyGlyGlySer)x3 linker
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shLuc
Plasmid#83092PurposeLentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)DepositorInsertshLuc
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shGFP
Plasmid#83085PurposeLentiviral shRNA vector for inducible knockdown of GFP (control hairpin)DepositorInsertshGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shLacZ
Plasmid#98632PurposeLentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)DepositorInsertshLacZ
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6A-EGFR ECD (1-644)
Plasmid#42666DepositorInsertEGFR ECD (1-644) (EGFR Human)
UseTags6xHis and MycExpressionMammalianMutationPromoterAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-GST-CD(Cbx7)
Plasmid#82525PurposeExpresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 chromodomain only; amino acids 1–62.DepositorInsertChromobox Homolog 7 (CBX7 Human)
UseTagsGSTExpressionBacterialMutationCD(Cbx7) ; amino acids 1–62PromoterAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-GST-ATL(Cbx7)
Plasmid#82526PurposeExpresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 ATL region only, amino acids 66–81.DepositorInsertChromobox Homolog 7 (CBX7 Human)
UseTagsGSTExpressionBacterialMutationATL(Cbx7), amino acids 66–81PromoterAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shSMAD4 #2
Plasmid#83091PurposeLentiviral shRNA vector for inducible knockdown of human SMAD4DepositorInsertshSMAD4 (SMAD4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shId2 #2
Plasmid#83090PurposeLentiviral shRNA vector for inducible knockdown of mouse Id2DepositorInsertshId2 (Id2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shGDF11 #2
Plasmid#83084PurposeLentiviral shRNA vector for inducible knockdown of human GDF11DepositorInsertshGDF11 (GDF11 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shID2 #2
Plasmid#83087PurposeLentiviral shRNA vector for inducible knockdown of human ID2DepositorInsertshID2 (ID2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-GST-CD-ATL (Cbx7)
Plasmid#82527PurposeExpresses mutant GST-CD-ATL (Cbx7) fusion proteins in E. coli. Cbx7 CD-ATL region, amino acids 1–84.DepositorInsertChromobox Homolog 7 (CBX7 Human)
UseTagsGSTExpressionBacterialMutationamino acids 1–84PromoterAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-GST-CD-ATLm(Cbx7)
Plasmid#82528PurposeExpresses mutant GST-CD-ATLm (Cbx7) fusion proteins in E. coli. Cbx7 amino acids 1-84 with mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.DepositorInsertChromobox Homolog 7 (CBX7 Human)
UseTagsGSTExpressionBacterialMutationamino acids 1-84 with mutation of RKR70-72, K75, …PromoterAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDNR-mCherry-T2A-3xPuroR-Frame 1
Plasmid#206992PurposeDonor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 1-13 KR
Plasmid#129299PurposeExpresses pCMV 3xFLAG-ATG3 1-13 KR (first thirteen lysines mutated to arginine) in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged first 13 Lysine residues to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
SIAP-1-COMP-blac-flag-his
Plasmid#111002PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertsporozoite invasion associated protein 1 (SIAP-1) (PF3D7_0408600 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2
Plasmid#192183PurposeClone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2DepositorInsertClone 2 heavy chain (HH02)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRAIP-SFB
Plasmid#101769PurposeMammalian expression of TRAIP fusion proteinDepositorInsertTRAIP / RNF206 (TRAIP Human)
UseTagsS peptide-flag-streptavidin binding peptide (SFB)ExpressionMammalianMutationsiRNA-resistant allelePromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ2042.2_GFPonly
Plasmid#140686Purpose1-color stripeDepositorInsert1-color stripe
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ2048.2_Gs
Plasmid#140672Purpose1-color stripe (“GREEN”)DepositorInsert1-color stripe (“GREEN”)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ2042.2_Bs
Plasmid#140671Purpose1-color stripe (“BLUE”)DepositorInsert1-color stripe (“BLUE”)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
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CL20_mEGFP_TSG101_YWHAB
Plasmid#205948PurposeExpress mEGFP-tagged fusion protein, TSG101_YWHAB from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PTK2_PSMA7
Plasmid#205904PurposeExpress mEGFP-tagged fusion protein, PTK2_PSMA7 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_THBS1_FSIP1
Plasmid#205940PurposeExpress mEGFP-tagged fusion protein, THBS1_FSIP1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ2042.2
Plasmid#140670Purpose3-color stripeDepositorInsert3-color stripe
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA RBD-GFP11
Plasmid#133069PurposeExpresses RBD domain with tripartite split GFP11 (M4)DepositorInsertRBD (Rhotekin-Rho Binding Domain)
UseTagsGFP11 (tripartite split-GFP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ2042.2_invRep
Plasmid#140688Purpose3-color stripe (inverted reporters)DepositorInsert3-color stripe (inverted reporters)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only