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Showing: 41 - 60 of 6765 results

  1. pRSET a sfGCaMP6s-T78H

    Plasmid
    #100023
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s-T78H
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100023-plasmid-map-sequence-id-194290

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  2. pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid
    #100050
    Purpose
    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    hChR2(E123T/H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized, E123T/H134R
    Promoter
    CaMKIIa
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100050-plasmid-map-sequence-id-194451

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  3. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670)

    Plasmid
    #100061
    Purpose
    AAV expression of ChR1-VChR1 Chimera fused to mCherry driven by Ef1a promoter
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    EF1a
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100061-plasmid-map-sequence-id-194678

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  4. pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid
    #100049
    Purpose
    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by hSynap promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    hChR2(E123T/H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized, E123T/H134R
    Promoter
    hSynap
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100049-plasmid-map-sequence-id-194448

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  6. pOD2049-csnControl

    Plasmid
    #100007
    Purpose
    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2048-csnDEG. Expression is controlled by Posm-6 (ciliated sensory neuron specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Inserts
    zif-1 (zif-1 Nematode)
    unc-119
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    100007-plasmid-map-sequence-id-193784

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  7. pMT3-RhoGC

    Plasmid
    #100031
    Purpose
    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.
    Depositor
    Daniel Oprian
    Article
    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1
    Insert
    RhoGC
    Use
    Tags
    1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)
    Expression
    Mammalian
    Mutation
    Promoter
    Adenovirus major late promoter
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    100031-plasmid-map-sequence-id-192905

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  9. pRSET a sfMatryoshCaMP6s

    Plasmid
    #100020
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100020-plasmid-map-sequence-id-194297

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  10. pET28-his-3C_Clu3B6p

    Plasmid
    #100093
    Purpose
    expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide
    Depositor
    Nikolai Sluchanko
    Article
    Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10
    Insert
    14-3-3 sigma human (SFN Human)
    Use
    Tags
    HSPB6 peptide and His6
    Expression
    Bacterial
    Mutation
    75-77 residues changed to AAA to facilitate cryst…
    Promoter
    T7
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100093-plasmid-map-sequence-id-193872

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  11. pMT3-RhoAC

    Plasmid
    #100032
    Purpose
    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with adenylyl cyclase activity.
    Depositor
    Daniel Oprian
    Article
    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1
    Insert
    RhoAC
    Use
    Tags
    1D4 (TETSQVAPA)
    Expression
    Mammalian
    Mutation
    E497K/C566D
    Promoter
    Adenovirus major late promoter
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    100032-plasmid-map-sequence-id-193105

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  12. pMT3-RhoGC E254D

    Plasmid
    #100033
    Purpose
    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.
    Depositor
    Daniel Oprian
    Article
    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1
    Insert
    RhoGC E254D
    Use
    Tags
    1D4 (TETSQVAPA)
    Expression
    Mammalian
    Mutation
    E254D
    Promoter
    Adenovirus major late promoter
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  17. pMT3-RhoGC D380E

    Plasmid
    #100035
    Purpose
    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.
    Depositor
    Daniel Oprian
    Article
    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1
    Insert
    RhoGC D380E
    Use
    Tags
    1D4 (TETSQVAPA)
    Expression
    Mammalian
    Mutation
    D380E
    Promoter
    Adenovirus major late promoter
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    100035-plasmid-map-sequence-id-192913

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  18. pMT3-RhoGC D380N

    Plasmid
    #100034
    Purpose
    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.
    Depositor
    Daniel Oprian
    Article
    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1
    Insert
    RhoGC D380N
    Use
    Tags
    1D4 (TETSQVAPA)
    Expression
    Mammalian
    Mutation
    D380N
    Promoter
    Adenovirus major late promoter
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    100034-plasmid-map-sequence-id-193102

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Showing: 41 - 60 of 6765 results