We narrowed to 140 results for: Cas3

1. pSEVA63-GG

   Plasmid

   #208015

   Purpose

   for cloning homology arms, with GFP as counter selection marker

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Insert

   msfGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   14G

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA13-GG

   Plasmid

   #208002

   Purpose

   for cloning homology arms, with GFP as counter selection marker

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Insert

   msfGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   14G

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEVA43-GG

   Plasmid

   #208014

   Purpose

   for cloning homology arms, with GFP as counter selection marker

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Insert

   msfGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   14G

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSmart-EF1alpha-AcrIC8-NLS

   Plasmid

   #207395

   Purpose

   Expresses AcrIC8 with NLS in mammalian cells

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   EF1a-AcrIC8-bGH polyA
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSmart-EF1alpha-AcrIE2-NLS

   Plasmid

   #207396

   Purpose

   Expresses AcrIE2 with NLS in mammalian cells

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   EF1a-AcrIE2-bGH polyA
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSmart-EF1alpha-AcrIC9-NLS

   Plasmid

   #207397

   Purpose

   Expresses AcrIC9 with NLS in mammalian cells

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   EF1a-AcrIC9-bGH polyA
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b-TEV-AcrIC9

   Plasmid

   #207409

   Purpose

   Protein purification vector to produce AcrIC9 in E. coli

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   T7-TEV-AcrIC9
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSFDuet-3C-AcrIC8

   Plasmid

   #207410

   Purpose

   Protein purification vector to produce AcrIC8 in E. coli

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   T7-HRV3C-AcrIC8
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPV-EF1a-EGxxFP-iP-A

   Plasmid

   #204624

   Purpose

   Cloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSmart-EF1alpha-NlaCas7-p65

    Plasmid

    #207394

    Purpose

    Expresses NlaCas7-p65 activator fusion in mammalian cells

    Depositor

    Yan Zhang

    Article

    Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

    Insert

    EF1a-NlaCas7-p65-bGH polyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPV-C1-crRNA-cloning-EF1a-BA

    Plasmid

    #204623

    Purpose

    piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat

    Plasmid

    #178883

    Purpose

    Vector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    U6-Nla repeat-BbsI-Nla repeat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPV-EF1a-mRxxFP1(AfeI)-iP-A

    Plasmid

    #204625

    Purpose

    Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPV-EF1a-EGxxFP(DMDex45_10)-iP-A

    Plasmid

    #204621

    Purpose

    Single-strand annealing (SSA) vector for monitoring crRNA(DMDex45)'s activity. DMD intron 44 region (upstream of exon 45)

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A

    Plasmid

    #204622

    Purpose

    Single-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Insert

    mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA

    Plasmid

    #204620

    Purpose

    Expression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable.

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Insert

    crRNA#20 (targeting DMD intron44) and crRNA#23 (targeting DMD intron 55)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJEC619

    Plasmid

    #182955

    Purpose

    SYNZIP18-Cascade.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al ACS Synth Biol. 2022 Jan 25. doi: 10.102

    Insert

    SynZip18-Cascade
    

    Use

    Tags

    SynZip18

    Expression

    Bacterial

    Mutation

    SynZip18 fusion to CasA

    Promoter

    J23150

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSth_Cas6

    Plasmid

    #178779

    Purpose

    Expression of S. thermophilus type I-E cas6

    Depositor

    Chase Beisel

    Article

    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

    Insert

    S. thermophilus type I-E cas6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSth_Cas8

    Plasmid

    #178776

    Purpose

    Expression of S. thermophilus type I-E cas8

    Depositor

    Chase Beisel

    Article

    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

    Insert

    S. thermophilus type I-E cas8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSth_Cse2

    Plasmid

    #178777

    Purpose

    Expression of S. thermophilus type I-E cse2

    Depositor

    Chase Beisel

    Article

    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

    Insert

    S. thermophilus type I-E cse2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge