We narrowed to 10,367 results for: ESP

1. eSpCas9-plus

   Plasmid

   #126767

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, R1060A; amino acids 1005-1013 replaced wit…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9-EF-5Pou5f1

   Plasmid

   #174872

   Purpose

   CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)
   

   Use

   CRISPR

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-T3-eSpCAS9-NG-pA

   Plasmid

   #131466

   Purpose

   Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

   Insert

   Codon optimized eSpCas9-NG
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mPlum-CathespinB-6

   Plasmid

   #55955

   Purpose

   Localization: Lysosomes, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Insert

   CathespinB (Ctsb Rat)
   

   Tags

   mPlum

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. tetO-MESP1

   Plasmid

   #170694

   Purpose

   doxycycline-inducible overexpression of MESP1

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   MESP1 (MESP1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mTFP1-CathespinB-6

   Plasmid

   #55467

   Purpose

   Localization: Lysosomes, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   CathespinB
   

   Tags

   mTFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pIRESpuro2-UBXD1-3x HA

   Plasmid

   #31836

   Depositor

   Hemmo Meyer

   Article

   Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

   Insert

   UBXD1 (UBXN6 Human)
   

   Tags

   3x HA

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

8. KA2601_eSpCas9-G2P

   Plasmid

   #124203

   Purpose

   Expression of eSpCas9(1.1) and sgRNA

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mIFP12-sEspin-C-18

   Plasmid

   #56262

   Purpose

   Localization: Actin Bundling, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   sEspin
   

   Tags

   mIFP12

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. mApple-CathespinB-6

    Plasmid

    #54871

    Purpose

    Localization: Lysosomes, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    CathespinB
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

11. tdTomato-CathespinB-6

    Plasmid

    #58075

    Purpose

    Localization: Lysosomes, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    CathespinB (Ctsb Rat)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEmerald-sEspin-C-18

    Plasmid

    #54253

    Purpose

    Localization: Actin Bundling, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    sEspin
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. mEmerald-sEspin-N-18

    Plasmid

    #54254

    Purpose

    Localization: Actin Bundling, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    sEspin
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. mEmerald-CathespinB-6

    Plasmid

    #54024

    Purpose

    Localization: Lysosomes, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    CathespinB
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. EBFP2-CathespinB-6

    Plasmid

    #55236

    Purpose

    Localization: Lysosomes, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Article

    Sundaresan et al J Clin Oncol. 1987 Nov;5(11):1739-45.

    Insert

    CathespinB
    

    Tags

    EBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. mEos2-CathespinB-6

    Plasmid

    #57354

    Purpose

    Localization: Lysosomes, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    CathespinB (Ctsb Rat)
    

    Tags

    mEos2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. peSpCas9-J23109

    Plasmid

    #113152

    Purpose

    Plasmid constitutively expressing eSpCas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    eSpCas9
    

    Expression

    Bacterial

    Mutation

    K810A & K1003A & R1060A

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dronpa-CathespinB-6

    Plasmid

    #57265

    Purpose

    Localization: Lysosomes, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Insert

    CathespinB
    

    Tags

    Dronpa

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. peSpdCas9-J23109

    Plasmid

    #113153

    Purpose

    Plasmid constitutively expressing eSpdCas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    eSpdCas9
    

    Expression

    Bacterial

    Mutation

    D10A & K810A & H840A & K1003A & R…

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. mVenus-CathespinB-6

    Plasmid

    #56605

    Purpose

    Localization: Lysosomes, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Insert

    CathespinB
    

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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