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Plasmid#183565PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)DepositorInsertAttenuator Sequence 21
UseTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR{KDRT-RC}
Plasmid#182166PurposepENTR plasmid carrying yeast recombination site for Gateway cloning into introns of genes encoding fluorescent proteinsDepositorInsertKDRT
UsePentr plasmidTagsExpressionMutationPromoterAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR{KDRT}
Plasmid#182165PurposepENTR plasmid carrying yeast recombination site for Gateway cloning into introns of genes encoding fluorescent proteinsDepositorInsertKDRT
UsePentr plasmidTagsExpressionMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR
Plasmid#65091PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged sequence to render plasmid resistant to s…PromoterCMV, 2x TetAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestMYC_RBPMS_KDR
Plasmid#65667PurposeMYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsMYCExpressionMammalianMutationintroduced HindIII site to exchange FLAG-HA tag w…PromoterCMV, 2x TetAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_K100E
Plasmid#65093PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged lysine 100 to glutamic acid (KDR backgrou…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_F65A
Plasmid#65092PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchange phenylalanine 65 to alanine (KDR backbone)PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_K100A
Plasmid#65663PurposeFLAG-HA expression vector with RBPMS carrying mutation K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged lysine 100 to alanine (KDR background)PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_111del
Plasmid#65659PurposeFLAG-HA expression vector with RBPMS carrying deletion at amino acid 111 (1-111) and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationintroduced stop codon such as to produce deletion…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A
Plasmid#65660PurposeFLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged glutamic acid 97 to alanine, lysine 100 t…PromoterCMV, 2x TetAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_F27A
Plasmid#65661PurposeFLAG-HA expression vector with RBPMS carrying mutation F27A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged phenylalanine 27 to alanine (KDR backgrou…PromoterCMV, 2x TetAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2-kdrl:mCherry-caax
Plasmid#194287PurposeExpression plasmid for zebrafish or Danionella cerebrumDepositorInsertkdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)
UseZebrafish or danionella cerebrum expressionTagsmCherryCAAX from the Tol2kit (Kwan et al., 2007)ExpressionMutationPromoterkdrl(flk1)Available sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKDR-Lenti-TetOn
Plasmid#183555PurposepKDR compatible TetOn lentiviral transfer plasmidDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
360 pCR kdrb
Plasmid#13067DepositorInsertkdrb (kdr Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
KDR gRNA (BRDN0001146819)
Plasmid#77755Purpose3rd generation lentiviral gRNA plasmid targeting human KDRDepositorInsertKDR (KDR Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
KDR gRNA (BRDN0001146648)
Plasmid#77756Purpose3rd generation lentiviral gRNA plasmid targeting human KDRDepositorInsertKDR (KDR Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
KDR gRNA (BRDN0001144938)
Plasmid#77757Purpose3rd generation lentiviral gRNA plasmid targeting human KDRDepositorInsertKDR (KDR Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HA-Kdrl-3p-FokI-KK
Plasmid#35685DepositorInsertKdrl-3p-FokI-KK
UseMrna productionTagsHAExpressionMutationPromoterAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 shCavin1KDR-EGFP
Plasmid#187254PurposeLentiviral expression of shRNA targeting Cavin1 + reconstitution of mouse Cavin1 (Cavin1 rescue)DepositorInsertShRNA for Cavin1 (Cavin1 Mouse)
UseLentiviralTagsEGFPExpressionMutationPromoterU6, 5'LTRAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Kdr-mod
Plasmid#48177Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame elementDepositorInsert5" UTR of 2KDR (MC2R Human)
UseLuciferaseTagsExpressionMutationA/G MUTATION IN ATG OF UORFPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only