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pcDNA3.1 LIC 6A HIS-TEV-DPP8
Plasmid#166793PurposeMammalian cell expression vector for Flag-tagged DPP8DepositorInsertDPP8 (DPP8 Human)
UseTagsHIS-TEVExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP8
Plasmid#166795PurposeMammalian cell expression vector for FLAG-tagged DPP8DepositorInsertDPP8 (DPP8 Human)
UseTagsFLAG-TEVExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_K700E_hSF3B1
Plasmid#176591PurposeAdenoviral construct to change K to E at 700th amino acid (K700E) in human SF3B1DepositorInsertSF3B1 (SF3B1 Human)
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_FLAG_hSF3B1
Plasmid#176590PurposeAdenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1DepositorInsertSF3B1 (SF3B1 Human)
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA
Plasmid#139192PurposeExpresses C-terminal HA tagged wt human Mfn2 in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags3HAExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-SF3B1-WT
Plasmid#82576PurposeExpresses a codon-optimized ORF of human SF3B1 (wild-type)DepositorInserthuman SF3B1-WT (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
plko-shmfn2-mfn2-Rescue
Plasmid#160655Purpose"Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"DepositorInsertmfn2 (MFN2 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry N1 MFN2
Plasmid#157759PurposeExpression of MFN2:mcherry in cellsDepositorInsertMFN2 (MFN2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMYs-MFN2-IRES-mitoRFP
Plasmid#121997PurposeExpression of MFN2 and mitoRFP in mammalial cellsDepositorInsertMFN2-IRES-mitoRFP (MFN2 Human)
UseRetroviralTagsExpressionMutationsilent Q386Q Q387Q V388V Y389Y C390C E391E E392EPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-MFN2-Strep (SB253)
Plasmid#227602PurposeInducible expression of Strep-tagged MFN2 in Pichia pastorisDepositorInsertMFN2 (MFN2 Human)
UseTagsTwin-StrepTag, 10xHisExpressionYeastMutationPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL452-Sf3b1-K700E
Plasmid#90425Purposeselectable HDR vector to introduce K700E mutation within mus Sf3b1 gene (for use with sgSf3b1(T1) and pL452(hygro)-Sf3b1-K700K plasmids enabling generation of hemizygous K700E mutation)DepositorInsertSf3b1 - left and right homology arms (Sf3b1 Mouse)
UseHomology-directed repair vectorTagsExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-hSF3B1-K700E
Plasmid#82577PurposeExpresses a codon-optimized ORF of human SF3B1 ( K700E mutant)DepositorInserthuman SF3B1-K700E (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationK700EPromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLCO4A1
Plasmid#132236PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLCO4A1 (SLCO4A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1 Mfn2-HA S27A
Plasmid#139193PurposeExpresses C-terminal HA tagged human Mfn2 S27A in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags3HAExpressionMammalianMutationSerine 27 to AlaninePromoterCMV promoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA S27D
Plasmid#139194PurposeExpresses C-terminal HA tagged human Mfn2 S27D in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags2HAExpressionMammalianMutationSerine 27 to Aspartic AcidPromoterCMV promoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
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sgSf3b1(T1)
Plasmid#90424Purposeinduces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700DepositorInsertmus Sf3b1 gRNA (Sf3b1 Mouse)
UseCRISPR; Guiderna encoding for gene editingTagsExpressionMammalianMutationPromoterU6Available sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only
