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pCDNA3_V5-APEX2-Sec61B

Plasmid

#83412

Purpose

Expressed N-term tagged APEX2 on Sec61B in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

V5-APEX2-Sec61B (SEC61B Human, Synthetic)

Use

Tags

APEX2 and V5

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-Sec61β

Plasmid

#186574

Purpose

Expression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cells

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

SEC61B (SEC61B Human)

Use

Tags

FRB-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-SEC61B-mEGFP

Plasmid

#115910

Purpose

donor to insert EGFP at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-mEGFP (SEC61B Human)

Use

Donor template

Tags

EGFP

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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MCP-mCherry-SEC61B

Plasmid

#207671

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP) fused with ER resident protein SEC61B

Depositor

Christine Mayr

Article

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

SEC61B (SEC61B Human)

Use

Tags

MCP-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Sec61B

Plasmid

#120244

Purpose

Expresses V5-tagged Sec61B in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Sec61B (Sec61b Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFX-Venus-Sec61β

Plasmid

#174467

Purpose

Express Venus with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with endoplasmic reticulum-targeting sequence

Use

Tags

Sec61 subunit beta

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-SEC61B-mEGFP

Plasmid

#115921

Purpose

donor to insert mEGFP at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-mEGFP

Use

Donor template

Tags

EGFP

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-mCherry-Sec61β

Plasmid

#174468

Purpose

Express mCherry with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with endoplasmic reticulum-targeting sequence

Use

Tags

Sec61 subunit beta

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-SEC61B-dTomato

Plasmid

#115920

Purpose

donor to insert dTomato at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-dTomato

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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ECFP-FRB-Sec61b

Plasmid

#210290

Purpose

nucleus OM marker and "anchor" for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

ECFP-FRB-Sec61b

Use

Tags

ECFP and FRB

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-Sec61B

Plasmid

#207553

Purpose

Homologous recombination donor to integrate and expression cassette for EGFP-Sec61B in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-Sec61B

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 SNAP-Sec61B

Plasmid

#207580

Purpose

Homologous recombination donor to integrate and expression cassette for SNAPtag-Sec61B in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible SNAPTag-Sec61B

Use

Tags

SNAPTAG

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBoBi-hSEC61B-C-PEN2

Plasmid

#183648

Purpose

Lentiviral expression of a ER-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and SEC61B (PSENEN, SEC61B Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

WT

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-SECFP-Sec61β

Plasmid

#174465

Purpose

Express SECFP with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with endoplasmic reticulum-targeting sequence

Use

Tags

Sec61 subunit beta

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-Sirius-Sec61β

Plasmid

#174464

Purpose

Express Sirius with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with endoplasmic reticulum-targeting sequence

Use

Tags

Sec61 subunit beta

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-SEC61B-dTomato

Plasmid

#115913

Purpose

donor to insert dTomato at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-dTomato (SEC61B Human)

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-mTagBFP2-Sec61B

Plasmid

#228136

Purpose

Expresses mTagBFP2-Sec61B in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Sec61B (SEC61B Human)

Use

Retroviral

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Sec61β-Flag-APEX2-SOPP3

Plasmid

#229072

Purpose

Expresse APEX2-SOPP3 in ER in mammalian cell

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the ER (APEX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Sec61 beta pAcGFP-C1 (802)

Plasmid

#62008

Purpose

mammalian expression of GFP-Sec61 beta

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

Sec61 beta (SEC61B Human)

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pMRX-IZ-SEC61B-mCherry-FRB

Plasmid

#233349

Purpose

Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

SEC61B (SEC61B Human)
mCherry
FRB

Use

Retroviral

Tags

Expression

Mammalian

Mutation

T2098L mutant of FKBP-rapamycin binding domain of…

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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