We narrowed to 296 results for: actb

1. pBS-f1(+)-ACTB-mEGFP

   Plasmid

   #115909

   Purpose

   donor to insert EGFP at the ACTB locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   ACTB-mEGFP (ACTB Human)
   

   Use

   Donor template

   Tags

   EGFP

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97312

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HMEJ donor
   

   Use

   Lentiviral and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_ACTB-i1 sgRNA / hSpCas9

   Plasmid

   #172825

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOC4 Actb-mRuby3 KI

   Plasmid

   #183437

   Purpose

   FlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and mRuby3 donor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. actb2-lifeact-mKate2

   Plasmid

   #109750

   Purpose

   Ubiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequence

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   mKate2 fused to the actin binding lifeact sequence
   

   Use

   Zebrafish plasmids

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97310

   Purpose

   NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb NHEJ donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_rActb sgRNA / hSpCas9

   Plasmid

   #172833

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. hACTB-RFP-Kir7.1

   Plasmid

   #107179

   Purpose

   Expression of N-terminal fused Kir7.1 in mammalian cells.

   Depositor

   Bikash Pattnaik

   Insert

   KCNJ13 (KCNJ13 Human)
   

   Tags

   RFP

   Expression

   Mammalian

   Promoter

   hACTB

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-f1(-)-ACTB-iTagRFP-T

   Plasmid

   #115916

   Purpose

   donor to insert iTagRFP-T at the ACTB locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   ACTB-TagRFP-T
   

   Use

   Donor template

   Tags

   TagRFP-T

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pChd4 GGSG_mAID-3xF_BactBsd

    Plasmid

    #235508

    Purpose

    Targeting vector for Chd4-miniAID fusion, Roxed Bact-Bsd

    Depositor

    Brian Hendrich

    Insert

    Chd4 (Chd4 Mouse)
    

    Use

    Mouse Targeting

    Tags

    mAID, 3xFLAG

    Mutation

    None

    Promoter

    beta actin

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. psBIG1a ActBT2ATetP2AP - mAGH

    Plasmid

    #229973

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2ATetP2AP - mAGH
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. psBIG1a ActBT2AmcherryP2AP -TagBFPH

    Plasmid

    #229971

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2AmcherryP2AP -TagBFPH
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-ACTB-C200-T7

    Plasmid

    #229850

    Purpose

    Donor vector for the ACTB site containing T7 sequence for detection of HR products.

    Depositor

    Natsuko Chiba

    Insert

    Partial sequence of ACTB gene
    

    Use

    Hr donor vector for human actb gene.

    Available Since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHTC-HaloTag_ACTB:1-375

    Plasmid

    #210971

    Purpose

    Mammalian expression of full-length human ACTB. C-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    ACTB:M1-F375 (ACTB Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pHTN-HaloTag_ACTB:1-375

    Plasmid

    #210970

    Purpose

    Mammalian expression of full-length human ACTB. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    ACTB:M1-F375 (ACTB Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. MultiMate-HITI-2c ACTB

    Plasmid

    #206267

    Purpose

    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    

    Use

    CRISPR; Recombinant baculovirus production

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOC2 GFP-Actb KI

    Plasmid

    #183429

    Purpose

    CreOFF knock-in for GFP-beta-actin (amino acid position: before start codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330_ACTB-e1 sgRNA / hSpCas9

    Plasmid

    #172830

    Purpose

    Mammalian expression of a sgRNA targeting the exon 2 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330_ACTB-e2 sgRNA / hSpCas9

    Plasmid

    #172831

    Purpose

    Mammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330_ACTB-i5 sgRNA / hSpCas9

    Plasmid

    #172829

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge