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Showing: 41 - 60 of 246 results

  4. pODN-mR2-ACTB

    Plasmid
    #183868
    Purpose
    Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    ACTB homology arms with mRuby2-linker (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    183868-plasmid-map-sequence-id-362451

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  9. pX330-PITCh-ACTB

    Plasmid
    #207748
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    sgRNA Targeting N-terminus of ACTB (ACTB Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    207748-plasmid-map-sequence-id-414798

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  10. MultiMate-HITI-2c ACTB

    Plasmid
    #206267
    Purpose
    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    Use
    CRISPR; Recombinant baculovirus production
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    206267-plasmid-map-sequence-id-406623

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Showing: 41 - 60 of 246 results