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pMRBAD-link-CGFP

Plasmid

#52733

Purpose

L-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-CGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET11a-link-NGFP

Plasmid

#52732

Purpose

IPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-NGFP

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMRBAD-Z-CGFP

Plasmid

#52735

Purpose

L-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

Z+CGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-NGFP

Plasmid

#52734

Purpose

IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

NGFP+Z-fusion

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe-tetoff-mVenus-PICdc42

Plasmid

#91876

Purpose

PI(WT): Photo inhibitable (blue light sensitive)

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Use

Tags

mVenus

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-PA(WT)-Vav2

Plasmid

#86977

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/ZF (Vav2 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-uniRapR-P-Vav2(DPZ)

Plasmid

#91875

Purpose

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS2

Plasmid

#196978

Purpose

Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS2 tag

Use

Tags

Expression

Plant

Mutation

Promoter

pG10-90

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Use

Tags

Expression

Plant

Mutation

Promoter

pG10-90

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9 Null

Plasmid

#198727

Purpose

Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9

Use

Tags

Expression

Plant

Mutation

Promoter

pG10-90

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Fragment 1 Null

Plasmid

#198729

Purpose

Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

gRNA expression cassette in pENTR4

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(WT)-mCherry

Plasmid

#87356

Purpose

PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src (Src Mouse)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-PI(WT)-Vav2(DPZ)

Plasmid

#86974

Purpose

PI(DM): Photo-inhibitable/light sensitive; DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/C1 (Vav2 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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PI(LM)-Rac1(WT)dc1.FLARE

Plasmid

#87590

Purpose

PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
PBD
Rac1

Use

Tags

YPet and dTurq

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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PI(DM)-Rac1(WT)dc1.FLARE

Plasmid

#87589

Purpose

PI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
PBD
Rac1

Use

Tags

YPet and dTurq

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-MLG-EGFP-LST2

Plasmid

#220785

Purpose

Expresses N-terminal EGFP-LST2 in mammalian cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MLG-HMF-LST2

Plasmid

#220786

Purpose

Expresses N-terminal His10-Myc-FLAG-LST2 in mammalian cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Use

Tags

10xHis-Myc-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAceBAC2-MLG-HMF-LST2

Plasmid

#220787

Purpose

Expresses N-terminal His10-Myc-FLAG-LST2 in insect cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Use

Tags

10xHis-Myc-FLAG

Expression

Insect

Mutation

Promoter

p10

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Fragment1 with TLS2

Plasmid

#196983

Purpose

Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Intermediate fragment for assembly of gRNA-TLS2 construct

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA NIA Null

Plasmid

#198728

Purpose

Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AtNIA1 gRNAs

Use

Tags

Expression

Plant

Mutation

Promoter

pU6-26, pU6-29

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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