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pDR111_mannose_Klf4Gata-3

Plasmid

#188401

Purpose

Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Inserts

Klf4 (Klf4 Mouse)
Gata-3 (Gata3 Mouse)

Tags

PhoD secretion peptide

Expression

Bacterial

Mutation

See Depositor Comments Below

Promoter

D-mannose

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-14

Plasmid

#160714

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)

Use

Synthetic Biology

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-17

Plasmid

#160717

Purpose

TrpC Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

tryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-15

Plasmid

#160715

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X-FLAG peptide bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X-FLAG peptide

Use

Synthetic Biology

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-3

Plasmid

#160703

Purpose

tef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

tef1-alpha

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGAT

Plasmid

#112587

Purpose

Used for expression of protein in E. coli as Thrombin cleavable 6His-tag-GST fusions with a blunt end restriction site immediately after thrombin recognition sequence

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His-GST

Expression

Bacterial

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_GATA3_p.S140F

Plasmid

#81611

Purpose

Gateway Donor vector containing GATA3 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GATA3 (GATA3 Human)

Use

Gateway entry vector

Mutation

V3A; S140F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GATA3_p.R330K

Plasmid

#81410

Purpose

Gateway Donor vector containing GATA3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GATA3 (GATA3 Human)

Use

Gateway entry vector

Mutation

V3A; R330K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFUSB4_TGAT

Plasmid

#48595

Depositor

Stephen Ekker

Article

Ma et al PLoS One. 2013 May 29;8(5):e65259. doi:

Insert

RVD sequence: NG NN NI NG

Use

TALEN

Expression

Mammalian

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSB4_GGAT

Plasmid

#48531

Depositor

Stephen Ekker

Article

Ma et al PLoS One. 2013 May 29;8(5):e65259. doi:

Insert

RVD sequence: NN NN NI NG

Use

TALEN

Expression

Mammalian

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSB4_GATA

Plasmid

#48508

Depositor

Stephen Ekker

Article

Ma et al PLoS One. 2013 May 29;8(5):e65259. doi:

Insert

RVD sequence: NN NI NG NI

Use

TALEN

Expression

Mammalian

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSB4_GATC

Plasmid

#48509

Depositor

Stephen Ekker

Article

Ma et al PLoS One. 2013 May 29;8(5):e65259. doi:

Insert

RVD sequence: NN NI NG HD

Use

TALEN

Expression

Mammalian

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSB4_GATG

Plasmid

#48510

Depositor

Stephen Ekker

Article

Ma et al PLoS One. 2013 May 29;8(5):e65259. doi:

Insert

RVD sequence: NN NI NG NN

Use

TALEN

Expression

Mammalian

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSB4_GATT

Plasmid

#48511

Depositor

Stephen Ekker

Article

Ma et al PLoS One. 2013 May 29;8(5):e65259. doi:

Insert

RVD sequence: NN NI NG NG

Use

TALEN

Expression

Mammalian

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSB4_CGAT

Plasmid

#48467

Depositor

Stephen Ekker

Article

Ma et al PLoS One. 2013 May 29;8(5):e65259. doi:

Insert

RVD sequence: HD NN NI NG

Use

TALEN

Expression

Mammalian

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSB4_AGAT

Plasmid

#48403

Depositor

Stephen Ekker

Article

Ma et al PLoS One. 2013 May 29;8(5):e65259. doi:

Insert

RVD sequence: NI NN NI NG

Use

TALEN

Expression

Mammalian

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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II-TGAT

Plasmid

#43446

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

II-TGAT

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
II-GGAT

Plasmid

#43382

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

II-GGAT

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
II-GATA

Plasmid

#43359

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

II-GATA

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
II-GATC

Plasmid

#43360

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

II-GATC

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only