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bactin-loxp-BFP-loxp-dsred-pBSII

Plasmid

#68972

Purpose

for transgenic animals

Depositor

David Traver

Article

Kobayashi et al Nature. 2014 Aug 21;512(7514):319-23. do

Inserts

lox-BFP-loxp
dsred

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

Plasmid

#139484

Purpose

PCR template for mEGFP gene knock-in donor vector

Depositor

Kazuhiro Aoki

Article

Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

Insert

mEGFP-loxP-P2A-dTKneo-pA-loxP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDZ207 (loxP KAN loxP 24xPP7SL MDN1)

Plasmid

#35191

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

loxP KAN loxP 24xPP7SL MDN1

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pCLJ-F2P-LoxP-PGK-PuroR-LoxP

Plasmid

#227502

Purpose

Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Prdm8 promoter

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCLJ-F2P-LoxP-PGK-NeoR-LoxP

Plasmid

#227503

Purpose

Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Prdm8 promoter

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α-loxP-GCaMP6f-loxP-WPRE

Plasmid

#233288

Purpose

AAV vector with EF1α promoter and Cre-Out GCaMP6f, enabling to express GCaMP6f in Cre-negative cells.

Depositor

Dayu Lin

Article

Fang et al Neuron. 2018 Apr 4;98(1):192-207.e10. do

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

EF1α

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

Plasmid

#32702

Purpose

Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Insert

eGFP

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only

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EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

Plasmid

#198753

Purpose

Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity

Depositor

Jacco van Rheenen

Article

Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

Insert

EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

See depositor comments below

Promoter

EF1a

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

Plasmid

#158962

Purpose

Cre-dependet Cas9-GFP expression

Depositor

Michael Brand

Article

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Inserts

hsp70l promoter
floxed DsRed cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA

Plasmid

#113160

Purpose

A plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxP

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE

Plasmid

#32703

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

3x HA tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA

Plasmid

#155125

Purpose

Tol2 vector containing Cre-inducible QFGal4-switch

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4-switch

Use

Cre/Lox; Zebrafish expression

Tags

Expression

Mutation

Promoter

Ubi

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pXL-BacII-CAG-loxP-STOP-loxP-Venus

Plasmid

#170520

Purpose

Plasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )

Depositor

Joshua Sanes

Article

Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

Insert

CAG-loxP-STOP-loxP-Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

Plasmid

#158963

Purpose

Cre-dependet Cas9-GFP expression

Depositor

Michael Brand

Article

Kesavan et al Development. 2020 Jun 8;147(11). pii: de

Inserts

hsp70l promoter
floxed Non-FP cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP

Plasmid

#32145

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

loxP> STOP STOP loxP>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP

Plasmid

#121448

Purpose

To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations

Depositor

Brian Chen

Article

Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

Insert

RFPnols-PQR-ZeocinR (ACTB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDZ264 (pKAN tdtomato cyc loxP KAN loxP)

Plasmid

#35193

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

tdtomato cyc loxP KAN loxP

Use

Tags

Expression

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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pALS186 (ttTi5605-MCS loxP::C.br.unc-119(+)::loxP)

Plasmid

#129539

Purpose

modified pCFJ151 for C. elegans mosSCI at ttTi5605 or compatible sites; floxed C. br. unc-119(+) selection

Depositor

Arneet Saltzman

Article

Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

Type

Empty backbone

Use

Cre/Lox; Mos1-mediated single copy insertion (mos…

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

Plasmid

#227774

Purpose

Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

loxP-dsR-loxP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-sfGFP+HsbG syntron

Plasmid

#172477

Purpose

sfGFP with a synthetic intron derived from the human beta-globin gene

Depositor

Mario Capecchi

Insert

sfGFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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