-
Plasmid#227209PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationV485G; aa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
P273H USP7 catalytic
Plasmid#227208PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationP273H; aa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Y331H USP7 catalytic
Plasmid#227205PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationY331H; aa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
M225I USP7 catalytic
Plasmid#227206PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationM225I; aa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
USP7 N418S catalytic
Plasmid#227202PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationN418Sl; aa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L373F FL USP7
Plasmid#227220PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationL373FPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Y331H FL USP7
Plasmid#227218PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationY331HPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
P273H FL USP7
Plasmid#227221PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationP273HPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
M225I FL USP7
Plasmid#227219PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationM225IPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
K420E USP7 catalytic
Plasmid#227210PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationK420E; aa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
USP7 G392D catalytic
Plasmid#227203PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationG392D; aa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L469R FL USP7
Plasmid#227225PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationL469RPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
E345K USP7 catalytic
Plasmid#227211PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationE345K; aa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L373F USP7 catalytic
Plasmid#227207PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationL373F; aa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
G392A USP7 catalytic
Plasmid#227204PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationG392A; aa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
E345K FL USP7
Plasmid#227224PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationE345KPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
V485G FL USP7
Plasmid#227222PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationV485GPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest-894 (attR4-attR2)
Plasmid#161893PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coliDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-6
Plasmid#163132PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-4
Plasmid#163130PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only