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Plasmid#211471Purposelentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistanceDepositorInsertCas9_T2A_mNeonGreen_P2A_BlasticidinR
UseCRISPR and LentiviralTags3xFlagExpressionMammalianMutationPromoterhU6, EF-1aAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-mApple
Plasmid#193780PurposeTricistronic expression of Cas9-mApple-BSD in mammalian cellsDepositorInsertmApple
UseLentiviralTagsExpressionMammalianMutationPromoterIn frame with Cas9Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUDE710
Plasmid#103020Purposeexpression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)DepositorUseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-puro_Panc480-MT7
Plasmid#200941PurposeMultiplexed CRISPR array expressing 7 sgRNA in a lentiviral backboneDepositorInsertmultiplexed sgRNA array
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6, EF-1aAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
U17m
Plasmid#121023PurposeMoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.DepositorInsertBujard RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS26
Plasmid#32295DepositorTypeEmpty backboneUseBacterial gene additionTagsExpressionBacterialMutationPromoterrhaBAvailable sinceSept. 26, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHyo182
Plasmid#173136PurposeExpresses pheS_A294G in e.coliDepositorInsertpheS_A294G
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHyo245
Plasmid#173147PurposeExpresses pheS_A294G by dual T7 promoter in e.coliDepositorInsertpheS_A294G
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pStA1CZ
Plasmid#114166PurposeStart-Stop Assembly Level 1CZ plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStA212
Plasmid#114171PurposeStart-Stop Assembly Level 212 plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRoRH
Plasmid#213133PurposeRiboptoregulator circuit RoRH for blue-light-activated gene expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS34
Plasmid#32297DepositorInsertlacZ ORF with long 5'UTR
UseBacterial gene additionTagsExpressionBacterialMutationPromoterrhaBpAvailable sinceOct. 18, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMS33
Plasmid#32296DepositorInsertlacZ ORF with short 5'UTR
UseBacterial gene additionTagsExpressionBacterialMutationPromoterrhaBpAvailable sinceOct. 18, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRoRL
Plasmid#213132PurposeRiboptoregulator circuit RoRL for blue-light-activated gene expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pStA0::ECK120015170
Plasmid#171879PurposeTerminator ECK120015170 in pStA0DepositorInsertTerminator ECK120015170
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-mNeonGreen
Plasmid#124102PurposeExpression of the fluorescent protein mNeonGreen in mammalian cellsDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationCodon usage was optimized for Homo SapiensPromoterUbCAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStA1DZ
Plasmid#114168PurposeStart-Stop Assembly Level 1DZ plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStA1DE
Plasmid#114169PurposeStart-Stop Assembly Level 1DE plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStA1EZ
Plasmid#114170PurposeStart-Stop Assembly Level 1EZ plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStA223
Plasmid#114172PurposeStart-Stop Assembly Level 223 plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only