Addgene

We narrowed to 1,991 results for: rp1

Showing: 581 - 600 of 1991 results

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YIplac204-pOst1-ESCargo(Orange)

Plasmid

#140157

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Plasmid

#140155

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#115428

Purpose

Expresses a non-glycosylated regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA2

Plasmid

#26678

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 2 (KPNA2 Human)

Use

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pLEX307 ASC

Plasmid

#163977

Purpose

Mammalian expression vector encoding ASC (for example, to assess ASC-speck formation or inflammasome signalling assays)

Depositor

Daniel Bachovchin

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

ASC (PYCARD Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA-trp-HYB

Plasmid

#64331

Purpose

trp1 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of trp1 deletion gRNA cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
p404-BrdU-Inc

Plasmid

#71790

Purpose

BrdU-incorporating plasmid with selectable marker TRP1

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1 and GPD

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TEV-GFP-FLAG LIC cloning vector (6LD)

Plasmid

#166835

Purpose

Modified LIC 6D backbone with a C-terminal TEV-GFP-FLAG tag. Derived from Scott Gradia's pcDNA3.1 LIC 6D by Liron David.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A CARD8 CARD-FLAG WT

Plasmid

#164020

Purpose

Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hArfaptin2

Plasmid

#206405

Purpose

Mammalian expression of hArfaptin2

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin2 (ARFIP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 UPA-CARD WT

Plasmid

#164021

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac204-TDH3p-LDH-CYC1t

Plasmid

#216121

Purpose

Yeast integrative vector for constitutive expression of LDH at the TRP1 locus

Depositor

Yvonne Nygård

Article

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Lactate dehydrogenase (LDH Bos taurus, Bovine)

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3 promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR/zeo-MACROD1

Plasmid

#172572

Purpose

For gateway cloning of N-terminal tagged MACROD1 constructs.

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-hArfaptin1

Plasmid

#206402

Purpose

Mammalian expression of hArfaptin1

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin1 (ARFIP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-hArfaptin2

Plasmid

#206403

Purpose

Mammalian expression of hArfaptin2

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin2 (ARFIP2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/MACROD1

Plasmid

#172578

Purpose

For generation of FlpIn lines with untagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD WT

Plasmid

#164022

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARD

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 UPA-STOP

Plasmid

#164031

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pTJK518

Plasmid

#121475

Purpose

CEN TRP1 CNA1ΔAID

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNA1deltaAID

Use

Yeast cen vector

Tags

Expression

Mutation

Truncated after amino acid 508

Promoter

Endogenous genomic promoter region

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK519

Plasmid

#121476

Purpose

CEN TRP1 CNA1ΔCBD

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNA1deltaCBD

Use

Yeast cen vector

Tags

Expression

Mutation

Truncated after amino acid 453

Promoter

Endogenous genomic promoter region

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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