We narrowed to 1,731 results for: lin tian

1. pRL056 pDESTattB_exorh:EGFP

   Plasmid

   #213510

   Purpose

   attB-containing pDEST plasmid, for use in Multisite Gateway reaction

   Depositor

   Christian Mosimann

   Article

   Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSEM238

   Plasmid

   #161515

   Purpose

   Neuronal expression of histamine-sensitive chloride channel for negative selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

   Insert

   pEXP[Psnt-1 | histamine | rpl-3 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Psnt-1

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCK123 pDESTattB_exorh:mCerulean

   Plasmid

   #213512

   Purpose

   attB-containing pDEST plasmid, for use in Multisite Gateway reaction

   Depositor

   Christian Mosimann

   Article

   Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTORGm43GWY

   Plasmid

   #112906

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

   Type

   Empty backbone

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. OmpT

   Plasmid

   #213137

   Purpose

   OmpT_native membrane expression

   Depositor

   Sebastian Hiller

   Article

   Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

   Insert

   OmpT
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTORGm43GWR

   Plasmid

   #112905

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

   Type

   Empty backbone

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTORGm43GWC

   Plasmid

   #112903

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

   Type

   Empty backbone

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTORGm43GWF

   Plasmid

   #112904

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mWasabi.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

   Type

   Empty backbone

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRVdG-4mCherry

   Plasmid

   #52488

   Purpose

   Expresses mCherry

   Depositor

   Sebastian Seung

   Article

   Weible et al J Neurosci. 2010 Dec 8;30(49):16509-13.

   Insert

   mCherry
   

   Use

   Rabies virus

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRVdG-4ChopV

    Plasmid

    #52476

    Purpose

    Expresses ChopV

    Depositor

    Sebastian Seung

    Article

    Kiritani et al J Neurosci. 2012 Apr 4;32(14):4992-5001.

    Insert

    ChopV
    

    Use

    Rabies virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICH37326

    Plasmid

    #50307

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    signal peptide, NbCRT calreticulin (Nicotiana benthamiana)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDestattB/CY (pCM327)

    Plasmid

    #68341

    Purpose

    Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.

    Depositor

    Leonard Zon, Christian Mosimann

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Type

    Empty backbone

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

    Plasmid

    #159859

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

    Plasmid

    #159849

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

    Plasmid

    #182351

    Purpose

    Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

    Plasmid

    #182358

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Pmex-5

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

    Plasmid

    #159872

    Purpose

    Three-fragment Gateway compatible protein tag for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159847

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

    Plasmid

    #159846

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

    Plasmid

    #159866

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

    Plasmid

    #159874

    Purpose

    Three-fragment Gateway compatible protein tag for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159869

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEN-R2-mCsfGFP-L3

    Plasmid

    #178602

    Purpose

    It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

    Depositor

    Frederica Theodoulou

    Article

    Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

    Insert

    Tandem fluorescent protein timers (tFTs)
    

    Use

    Entry vector for gateway cloning

    Tags

    mCherry superfolder GFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159855

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

    Plasmid

    #159873

    Purpose

    Three-fragment Gateway compatible protein tag for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCFJ2241 - [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159860

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

    Plasmid

    #159864

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159851

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159843

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Lneg-INTR-GFP

    Plasmid

    #208902

    Purpose

    encodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius neglectus, tagged with GFP

    Depositor

    Christian Gruber

    Article

    Liutkeviciute et al FASEB J. 2018 Jun 25;32(12):fj201800443.

    Insert

    INTR
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

    Plasmid

    #159844

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159865

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HA-Foxo1ADA (pCMV5)

    Plasmid

    #12143

    Depositor

    Domenico Accili

    Article

    Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

    Insert

    Foxo1 ADA (Foxo1 Mouse)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Phosphorylation-deficient, also referred to as co…

    Promoter

    CMV

    Available since

    Aug. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

    Plasmid

    #159817

    Purpose

    Positive control for germline GFP expression from simple array in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPB-MPP8-112–858_Flag2-Blast

    Plasmid

    #175039

    Purpose

    Mammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 tag

    Depositor

    Kristian Helin

    Article

    Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

    Insert

    Mphosph8 (Mphosph8 Mouse)
    

    Use

    Piggybac

    Tags

    Flag2

    Expression

    Mammalian

    Mutation

    Expression of MPP8 protein from amino acid positi…

    Promoter

    CAG

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPB-MPP8-1–729_Flag2-Blast

    Plasmid

    #175040

    Purpose

    Mammalian expression of mMPP8 (M1 to E729) with C-terminal Flag2 tag

    Depositor

    Kristian Helin

    Article

    Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

    Insert

    Mphosph8 (Mphosph8 Mouse)
    

    Use

    Piggybac

    Tags

    Flag2

    Expression

    Mammalian

    Mutation

    Expression of MPP8 protein from amino acid positi…

    Promoter

    CAG

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPB-MPP8-∆ARD_Flag2-Blast

    Plasmid

    #175041

    Purpose

    Mammalian expression of mMPP8 (deletion from D570 to K718) with C-terminal Flag2 tag

    Depositor

    Kristian Helin

    Article

    Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

    Insert

    Mphosph8 (Mphosph8 Mouse)
    

    Use

    Piggybac

    Tags

    Flag2

    Expression

    Mammalian

    Mutation

    Expression of MPP8 protein with deletion of the a…

    Promoter

    CAG

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET21a-LysRS-His

    Plasmid

    #124114

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Lysine-tRNA ligase (lysU Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pQE30-His-AlaRS

    Plasmid

    #124103

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Alanyl-tRNA ligase (alaS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET21a-AspRS-His

    Plasmid

    #124106

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Aspartate-tRNA ligase (aspS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET21a-ProRS-His

    Plasmid

    #124117

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Prolyl-tRNA ligase (proS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET21a-TyrRS-His

    Plasmid

    #124121

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tyrosyl-tRNA ligase (tyrS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET21a-ValRS-His

    Plasmid

    #124122

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Valyl-tRNA ligase (valS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. mTurquoise2

    Plasmid

    #198196

    Purpose

    Spectral correction: Expression of mTurquoise2

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAAV-RSV-SpCas9

    Plasmid

    #85450

    Purpose

    Express SpCas9 in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pRSV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pRSV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET-21a(+)-IPP1-His

    Plasmid

    #118978

    Purpose

    Bacterial expression plasmid for Inorganic pyrophosphatase

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    inorganic diphosphatase IPP1 (IPP1 Budding Yeast)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET21a-GluRS-His

    Plasmid

    #124109

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutamyl-tRNA ligase (gltX Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET21a-GlnRS-His

    Plasmid

    #124108

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutaminyl-tRNA ligase (glnS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET21a-SerRS-His

    Plasmid

    #124118

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Seryl-tRNA ligase (serS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pQE60-EFG-His

    Plasmid

    #124126

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Elongation factor G (fusA Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge