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Showing: 1451 - 1500 of 1842 results
  1. pHS33

    Plasmid
    #171202
    Purpose
    bacterial experssion of a GST-tagged MEG-3 C-terminus with mutations in the HMG-like motif
    Depositor
    Insert
    meg-3 (meg-3 Nematode)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    aa545-862, F705A,Y708A,Y713A, N725A
    Promoter
    Available since
    Aug. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pAAP21

    Plasmid
    #171207
    Purpose
    bacterial expression for HIS purification of the MEG-3 C-terminus with mutations in the HMG-like motif
    Depositor
    Insert
    meg-3 (meg-3 Nematode)
    Use
    Tags
    8xHIS
    Expression
    Bacterial
    Mutation
    aa545-862, F705A,Y708A,Y713A, N725A
    Promoter
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pHS29

    Plasmid
    #171205
    Purpose
    bacterial expression for HIS purification of MEG-3 with mutations in the HMG-like motif
    Depositor
    Insert
    meg-3 (meg-3 Nematode)
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    F705A,Y708A,Y713A, N725A
    Promoter
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pSMT3-FBF2 SS/R

    Plasmid
    #166976
    Purpose
    Expresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Insert
    FBF-2 (fbf-2 Nematode)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pSMT3-FBF2 SS/Y

    Plasmid
    #166973
    Purpose
    Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Insert
    FBF-2 (fbf-2 Nematode)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pSMT3-FBF2 AS/Y

    Plasmid
    #166974
    Purpose
    Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Insert
    FBF-2 (fbf-2 Nematode)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pSMT3-FBF2 AQ/Y

    Plasmid
    #166975
    Purpose
    Expresses FBF-2 with AQ/Y mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Insert
    FBF-2 (fbf-2 Nematode)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. KG#797

    Plasmid
    #110916
    Purpose
    Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons
    Depositor
    Inserts
    unc-129 promoter
    STRN-1 (Sentryn)-GFP
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    Use
    Tags
    GFP (contains 3 artificial introns)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. KG#471

    Plasmid
    #110935
    Purpose
    Expresses proteins in a subset of neurons in C. elegans, including isolated expression in the DA9 cholinergic motor neuron in the tail
    Depositor
    Inserts
    mig-13 promoter
    unc-54 3' control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. KG#920

    Plasmid
    #110937
    Purpose
    Expresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animal
    Depositor
    Inserts
    mig-13 promoter
    INS-22-Emerald
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid
    #159852
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Insert
    [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)

    Plasmid
    #159865
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Insert
    [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

    Plasmid
    #159838
    Purpose
    Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays
    Insert
    pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1451 - 1500 of 1842 results