We narrowed to 8,244 results for: 11
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Plasmid#121942PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterhuman U6 promoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pdCas9-DNMT3A-PuroR (ANV)
Plasmid#71684PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-nucGFP11-3xControlgRNA
Plasmid#224568PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)MutationCodon optimizedAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR
Plasmid#171240PurposeMammalian expression of 3xFlag-tagged human androgen receptor (hAR)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xFlag-tagged human androgen receptor (AR Human)
TagsFlagExpressionMammalianPromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DHFR-SpCas9-DHFR
Plasmid#85447PurposeExpresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cellsDepositorInsertDHFR-SpCas9-DHFR
UseAAVTagsDestabilized domain (C-terminus version) of E.col…ExpressionMammalianPromoterCbhAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR-IAA4_no stop
Plasmid#83866PurposeGateway donor vector for IAA4 with no stop codonDepositorAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
HSD11B1-FLAG
Plasmid#24096DepositorInsertHSD11beta1 (HSD11B1 Human)
UseLentiviralAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
CIB1-CreC(N1)
Plasmid#75367PurposeExpresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCIB1 (CIB1 Mustard Weed)
TagsCre DNA recombinase, residues 106-343ExpressionMammalianPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p026 pCS2-EnR
Plasmid#11028DepositorInsertengrailed (en Fly)
UseXenopus, mammalian, avian, and zebrafishMutationEngrailed repressor domain (bases encoding amino …Available sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-EFS-PCP-GFPnls
Plasmid#121938PurposeCRISPR-Sirius plasmidDepositorInsertPCP-GFPnls
UseLentiviralTagssfGFPExpressionMammalianPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJRH-1180 U6-reci Gag-pol v2
Plasmid#201915PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-pol
ExpressionMammalianPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p3xFLAG-CMV-Tlr4
Plasmid#27148DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttoll-like receptor 4 (Tlr4 Mouse)
Tags3x FLAGExpressionMammalianMutationNative signal peptide sequence (original amino ac…Available sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
C-BLa 1.1
Plasmid#88997PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.1 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG FUCCI
Plasmid#136934Purposedonor plasmid for constitutive FUCCI expression in human cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
UseSynthetic BiologyTagsClover and mKO2ExpressionMammalianPromoterCAGAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-TO-dCas9-P2A-HSA
Plasmid#121936PurposeCRISPR-Sirius plasmidDepositorInsertdCas9-P2A-HSA
UseCRISPR and LentiviralTagsHSAExpressionMammalianPromoterCMV-TOAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-F-API5
Plasmid#157662PurposeExpresses API5 in mammalian cellDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertApoptosis inhibitor 5 (API5 Human)
TagsFLAG tagExpressionMammalianAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Flag-cMyc T58A
Plasmid#20075DepositorAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET15b-RvLEAMshort
Plasmid#218122PurposeExpresses truncated RvLEAM protein under IPTG induction in bacterial host cells.DepositorInsertRvLEAMshort
Tags6xHISExpressionBacterialMutationTruncated form (58-181aa)PromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4.23_Mmp11
Plasmid#209520PurposeExpresses luciferase under control of Mmp11 promoter in transfected cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMmp11 promoter (Mmp11 Mouse)
UseLuciferaseAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPUR-mU6-sgRNA-Sirius-8XPP7
Plasmid#121943PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianPromotermouse U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by