We narrowed to 1,197 results for: Aspa

1. pMBP-parallel1-GK5_v2 (D280N)

   Plasmid

   #134290

   Purpose

   Expresses GK5 (isoform 2)-D280N mutant in bacteria

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 280 to Asparagine

   Promoter

   T7

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMBP-parallel1-GK5_v2 (D30N)

   Plasmid

   #134289

   Purpose

   Expresses GK5 (isoform 2)-D30N mutant in bacteria

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 30 to Asparagine

   Promoter

   T7

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETDuet_huCAD_ICADL (TevS)

   Plasmid

   #100098

   Purpose

   dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

   Depositor

   Jim Wells

   Article

   Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

   Insert

   DNA fragmentation factor subunit alpha (CAD, DFFA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two caspase cleavage sites (DETD-117 and DAVD-224…

   Promoter

   T7

   Available since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIG-FGF13A

   Plasmid

   #220793

   Purpose

   To express the human FGF13 isoform 1(FGF13A) in mammalian cells together with EGFP

   Depositor

   Pierre Vanderhaeghen

   Article

   Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

   Insert

   FGF13 (FGF13 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-ASNS-OE-IRES-Thy1.1

   Plasmid

   #192947

   Purpose

   Asparagine synthetase overexpression in murine cells

   Depositor

   Ping-Chih Ho

   Article

   Fernandez-Garcia et al Cell Rep. 2022 Nov 15;41(7):111639. doi:

   Insert

   Asparagine synthetase (Asns Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   5'LTR

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

   Plasmid

   #129048

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA8 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

   Plasmid

   #129041

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA1 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. N295A (EcN), N229A (EcQ) MetNIQ in pBAD

   Plasmid

   #118581

   Purpose

   N295A Mutation of MetN in Methionine ABC Transporter and N229A Mutation in Methionine Binding Protein (MetQ) for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (metN E. coli)
   MetI (metI E. coli)
   MetQ (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala) and…

   Promoter

   None and araBAD Promotoer

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-GSDMD_H270A

   Plasmid

   #224701

   Purpose

   Expression of mutated Gasdermin D_H270A (GSDMD_H270A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_H270A (GSDMD Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H270A

   Promoter

   CMV

   Available since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA3.1-GSDMD_Q29A

    Plasmid

    #224702

    Purpose

    Expression of mutated Gasdermin D_Q29A (GSDMD_Q29A) in transfected mammalian cell lines

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD_Q29A (GSDMD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q29A

    Promoter

    CMV

    Available since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3.1-GSDMD_Q29A_H270A

    Plasmid

    #224703

    Purpose

    Expression of mutant Gasdermin D_Q29A_H270A (GSDMD_Q29A_H270A) in transfected mammalian cell lines

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD_Q29A_H270A (GSDMD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q29A and H270A

    Promoter

    CMV

    Available since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA3.1-GSDMD_D275A

    Plasmid

    #224704

    Purpose

    Expression of mutated Gasdermin D_D275A (GSDMD_D275A) in transfected mammalian cell lines

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD_D275A (GSDMD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D275A

    Promoter

    CVM

    Available since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pcDNA3.1-GSDMD_H270A_D275A

    Plasmid

    #224705

    Purpose

    Expression of mutated Gasdermin D_H270A_D275A (GSDMD_D275A) in transfected mammalian cell lines

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD_Q29A_H270A (GSDMD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H270A and D275A

    Promoter

    CMV

    Available since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pLX304-EZH1-D745N

    Plasmid

    #203585

    Purpose

    Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.

    Depositor

    Abhishek Chakraborty, William Kaelin

    Article

    Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

    Insert

    EZH1 (EZH1 Human)
    

    Use

    Lentiviral

    Tags

    V5-tagged

    Expression

    Mammalian

    Mutation

    changed Aspartic Acid 745 to Asparagine for parti…

    Promoter

    CMV

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Myc-Nix-S212D

    Plasmid

    #197515

    Purpose

    Mammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-212. Based on Myc-Nix (#100795)

    Depositor

    Joseph Gordon

    Article

    da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

    Insert

    BCL2 interacting protein 3 like (BNIP3L Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Serine 212 changed to aspartic acid

    Promoter

    CMV

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2iApowriter2(#336)

    Plasmid

    #184104

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transient transgenesis

    Tags

    internal tag: 5myc

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT22Apowriter2(#340)

    Plasmid

    #184105

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    internal tag: 5myc

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT22iuasvApoWtEos3N(#486)

    Plasmid

    #184107

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT22iuasvApoWChe(#488)

    Plasmid

    #184108

    Purpose

    cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

    Plasmid

    #91820

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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