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sgRNA with rpr-1 promoter

Plasmid

#48961

Purpose

to drive the sgRNA expression under RNase P non-coding RNA promoter

Depositor

Mario de Bono

Article

Chen et al Nucleic Acids Res. 2013 Sep 5.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

rpr-1 promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh1 shRNA

Plasmid

#122020

Purpose

Knock Down of Septin 11. It targetes Septin 11 mRNA. It knocks down all known splice variants of Sept 11 mRNAs.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-NQL007-NANOG-sgRNA

Plasmid

#175555

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

spCas9-nuclease and sgRNA against mouse NANOG STOP Codon

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 mouse twisted gastrulation

Plasmid

#25778

Depositor

Edward De Robertis

Insert

twisted gastrulation (Twsg1 Mouse)

Use

Tags

Flag and chordin signal peptide

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pENTR_Hu_CCNB2(WT)

Plasmid

#136341

Purpose

Mouse CCNB2 cDNA in Gateway pENTR

Depositor

Guillermo de Cárcer

Insert

CCCNB2

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Geph UTR shRNA

Plasmid

#121070

Purpose

shRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNA

Depositor

Angel de Blas

Article

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Cdc42 T17N sp64T

Plasmid

#16672

Depositor

De-Li Shi

Article

Djiane et al Development. 2000 Jul . 127(14):3091-100

Insert

Cdc42 T17N (CDC42 Human)

Use

Xenopus expression

Tags

Expression

Mutation

T17N. Dominant negative.

Promoter

Available since

April 28, 2008

Availability

Academic Institutions and Nonprofits only
p3xFLAG CMV14 / BRAF-D594E

Plasmid

#131714

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D594E

Promoter

CMV

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-gamma2/deltaILTM4

Plasmid

#119735

Purpose

GABAA receptor expression (chimeric rat rat gamma 2 subunit whose large intracellular loop, TM4 and C-term have been replaced with the equivalent domains of the rat delta subunit) C-myc near N-term

Depositor

Angel de Blas

Article

Wu et al J Biol Chem. 2012 Aug 10;287(33):27417-3

Insert

GABRG2/GABRD (Gabrg2, Gabrd Rat)

Use

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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PCDHGC5-mCherry

Plasmid

#119934

Purpose

Protocadherin gamma C5 expression (mCherry-tag at the C-terminus).

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_TetOn_Hu_PTTG1(WT)

Plasmid

#136346

Purpose

Lentiviral inducible expression (TetOn) of mouse PTTG1

Depositor

Guillermo de Cárcer

Insert

PTTG1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus sFRP2

Plasmid

#24696

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2000 Sep . 96(2):183-95.

Insert

secreted frizzled-related sequence protein 2 (sfrp2 Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST

Plasmid

#175018

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

OK-HEF s57a ED

Use

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

s57a esterase knock out mutant

Promoter

CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCD5-ss-D/bovine/France/5920/2014-HEFwtED-GCN4-sfGFP-ST

Plasmid

#175017

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

OK-HEFwtED

Use

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC FLAG hRap1 delta Myb

Plasmid

#13253

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Myb (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

contains hRap1 aa2-131 + 184-399 and the point mu…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
SEPT11 sh1 3m shRNA

Plasmid

#122021

Purpose

Negative control shRNA for SEPT11 sh1 shRNA (plasmid 122020).

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC TRF2 deltaB

Plasmid

#18006

Depositor

Titia de Lange

Article

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Expression

Mutation

Deleted Basic Domain (aa1-44)

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta CT

Plasmid

#13252

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta CT (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

contains hRap1 aa2-270 + 331-399 and the point mu…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta Br delta M

Plasmid

#13256

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br delta M (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

contains hRap1 aa2-44 + 184-399 and the point mut…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
gamma2 CR shRNA (shRNA2)

Plasmid

#120794

Purpose

shRNA targeting the coding region of the gamma2 mRNA

Depositor

Angel de Blas

Article

Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

Insert

GABRG2 shRNA (Gabrg2 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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