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pMIH-mRuby2-BAX

Plasmid

#111628

Purpose

Fluorescent fusion protein used to visualise mouse BAX, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

Bax (Bax Mouse)

Use

Retroviral

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMIH-mRuby2-BAK

Plasmid

#111627

Purpose

Fluorescent fusion protein used to visualise mouse BAK, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

BAK (Bak1 Mouse)

Use

Retroviral

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform2_Flag

Plasmid

#191999

Purpose

Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2 (LYSET Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMVTRE3G_LYSET-Isoform1_untagged-Puro

Plasmid

#192001

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform1 stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform1 (LYSET Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMCB320 sgCdkn1a.1

Plasmid

#227945

Purpose

sgRNA targeting Cdkn1a, mCherry, puromycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

Depositor

Laura Attardi

Article

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Cdkn1a.1 (Cdkn1a Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMCB320 sgCdkn1a.2

Plasmid

#227946

Purpose

sgRNA targeting Cdkn1a, mCherry, puromycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

Depositor

Laura Attardi

Article

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Cdkn1a.2 (Cdkn1a Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMVTRE3G_LYSET-Isoform2_untagged-Puro

Plasmid

#192002

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2 (LYSET Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMVTRE3G_LYSET-Isoform1_I142V-Puro

Plasmid

#192004

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform1 I142V mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform1 I142V (LYSET Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMVTRE3G_LYSET-Isoform1_R45W-Puro

Plasmid

#192003

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform1 R45W mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform1 R45W (LYSET Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMVTRE3G_LYSET-Isoform2-R45W-Flag

Plasmid

#192005

Purpose

Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 R45W mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2-R45W (LYSET Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-7xTRE-3xeGFP

Plasmid

#191206

Purpose

GFP expression under the control of TRE promotor

Depositor

Janelia Viral Tools

Article

Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

Insert

3X GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-pcDNA3.1 Puro-CAG-Voltron2

Plasmid

#172909

Purpose

Mammalian expression of voltage sensor

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Voltron2

Use

Tags

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDisplay-iSeroSnFR

Plasmid

#128484

Purpose

Fluorescent reporter for serotonin (mammalian expression, membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Use

Tags

Ig-kappa leader, Myc, PDGFR + enhanced ER export,…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iSeroSnFR-Nlgn

Plasmid

#128485

Purpose

Fluorescent reporter for serotonin (dendrite localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Has Service

AAV9

Insert

iSeroSnFR

Use

AAV

Tags

Full-length neuroligin, Ig-kappa leader, and cpsf…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEx-iSeroSnFR

Plasmid

#128486

Purpose

Fluorescent reporter for serotonin (conditional + membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Has Service

AAV9

Insert

iSeroSnFR

Use

AAV

Tags

Ig-kappa leader, Myc, PDGFR, and cpsfGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMVTRE3G_LYSET-Isoform2-I142V-Flag

Plasmid

#192006

Purpose

Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 I142V mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2-I142V-Flag (LYSET Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

Plasmid

#172910

Purpose

Mammalian expression of voltage sensor; soma localization targeting sequence

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Voltron2-ST

Use

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-FLEx-iSeroSnFR-EnhancedExport

Plasmid

#129180

Purpose

Fluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Use

AAV

Tags

Ig-kappa leader, Myc, and PDGFR + enhanced ER exp…

Expression

Mutation

Promoter

CAG

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-VARNAM A122D WPRE-bGH-polyA

Plasmid

#180486

Purpose

Mammalian expression of voltage sensor

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

VARNAM A122D

Use

Tags

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TRE-FRT-2xtTA

Plasmid

#191205

Purpose

Flpo dependent tTA expression under the control of TRE promotor

Depositor

Janelia Viral Tools

Article

Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

Insert

2xtTA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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