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pcDNA3-HA-GBF1

Plasmid

#206415

Purpose

Mammalian expression of hGBF1

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Paf1

Plasmid

#11059

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Paf1 (PAF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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ReNL/pcDNA3

Plasmid

#85203

Purpose

Red color variant of bright luminescent protein

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

Red enhanced Nano-lantern

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3/er-(n2)oxStayGold(c4)

Plasmid

#185822

Purpose

A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

Depositor

Atsushi Miyawaki

Article

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

er-(n2)oxStayGold(c4)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 - ciRS-7

Plasmid

#169139

Purpose

Expression of human CDR1as/ciRS-7 circRNA

Depositor

Jørgen Kjems, Thomas Hansen

Article

Hansen et al Nature. 2013 Mar 21;495(7441):384-8. doi

Insert

CDR1as/ciRS-7 (CDR1-AS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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hJAM-A pcDNA3.1

Plasmid

#70073

Purpose

Expresses human junctional adhesion molecule-A in mammalian cells

Depositor

Terence Dermody

Article

Danthi et al J Virol. 2006 Feb;80(3):1261-70.

Insert

junctional adhesion molecule-A (F11R Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Nanog

Plasmid

#28221

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Jan 18. ():.

Insert

Nanog (NANOG Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TRAIL-R2

Plasmid

#61383

Purpose

Expresses TRAIL receptor 2

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 2 (TNFRSF10B Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Beclin1

Plasmid

#21150

Depositor

Junying Yuan

Article

Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

Insert

PcDNA3-Beclin1 (BECN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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Twitch-2B pcDNA3

Plasmid

#49531

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2B

Use

Tags

ECFP and cpCit174

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3_NSnoFbox-vhhGFP4

Plasmid

#35580

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSnoFbox-vhhGFP4 (slmb Fly)

Use

Tags

Expression

Mammalian

Mutation

F box domain of slmb deleted.

Promoter

Available since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean(1-10)

Plasmid

#165144

Purpose

Expresses Cerulean(1-10) in mammalian cells

Depositor

Daichi Kamiyama

Article

Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

Insert

Cerulean(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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HA-p73α-pcDNA3

Plasmid

#22102

Depositor

William Kaelin

Article

Jost et al Nature. 1997 Sep 11. 389(6647):191-4.

Insert

tumor protein p73 (TP73 Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Promoter

Available since

Sept. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRARγ.hRXRα

Plasmid

#135416

Purpose

Human Retinoic Acid Receptor-gamma and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Inserts

Human retinoic acid receptor-gamma
Human retinoid X receptor-alpha

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and SV40

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRARβ.hRXRα

Plasmid

#135415

Purpose

Human Retinoic Acid Receptor-beta and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Inserts

Human retinoic acid receptor-beta
Human retinoid X receptor-alpha

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and SV40

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA_DENV2-NS2B3_V5

Plasmid

#115906

Purpose

Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

Depositor

Alan Rothman

Article

Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

Insert

DENV2 NS2B3 protease

Use

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TrxRFP1

Plasmid

#98996

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hSNCA-NE

Plasmid

#102361

Purpose

Overexpression of synuclein alpha in mammalian cells

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Insert

synuclein alpha (SNCA Human)

Use

Tags

NE

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRPV1exCellHalo

Plasmid

#220307

Purpose

Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

Depositor

Eric Senning

Article

Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

Insert

rTRPV1exCellHalo (Trpv1 Rat)

Use

Tags

circularly permutated Halotag (Deo et al., Nat Ch…

Expression

Mammalian

Mutation

linker included to insert cpHaloTag (

Promoter

CMV promoter

Available since

July 30, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 V5 hHR23A

Plasmid

#13053

Depositor

Steven Grossman

Article

Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

Insert

hHR23A (RAD23A Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

R325K and L355P relative to NP_001257291.1

Promoter

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

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