Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,182 results for: mcp

Showing: 581 - 600 of 1182 results

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pRSITEP-puro-shWRN2-C911

Plasmid

#125786

Purpose

a seed-matched control for pRSITEP-puro-shWRN2

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shWRN1

Plasmid

#125781

Purpose

constitutive expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1 (WRN Human)

Use

RNAi

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shWRN2

Plasmid

#125782

Purpose

constitutive expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgWRN1

Plasmid

#125771

Purpose

constitutive expression of a guide RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN1 (WRN Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shPSMD2

Plasmid

#125779

Purpose

constitutive expression of a short-hairpin RNA targeting human PSMD2 (RNAi positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shPSMD2 (PSMD2 Human)

Use

RNAi

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgWRN2

Plasmid

#125772

Purpose

constitutive expression of a guide RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN2 (WRN Human)

Use

CRISPR

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgCh2-2

Plasmid

#125775

Purpose

constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgCh2-2

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgWRN3

Plasmid

#125773

Purpose

constitutive expression of a guide RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN3 (WRN Human)

Use

CRISPR

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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CRaf-IRES-pErk reporter

Plasmid

#226170

Purpose

Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-CRaf-IRES-3xFlag-tdMCP-Erk2 (MAPK1, RAF1 Human)

Tags

3xFlag-tdMCP and Myc

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-bio-myc-cCE

Plasmid

#112711

Purpose

For tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Tags

HIV Rev nuclear localization signal (NES), Stop c…

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV with Tet repressor binding sites

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shRPS6

Plasmid

#125780

Purpose

constitutive expression of a short-hairpin RNA targeting human RPS6 (RNAi positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shRPS6 (RPS6 Human)

Use

RNAi

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgCh2-2

Plasmid

#125767

Purpose

constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgCh2-2

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgCh2-4

Plasmid

#125768

Purpose

constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgCh2-4

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-active WRN

Plasmid

#125788

Purpose

constitutive, ectopic expression of human active WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-active WRN (WRN Human)

Tags

5x myc

Expression

Mammalian

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN K577M

Plasmid

#125790

Purpose

constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

K577M (helicase-dead)

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN E84A

Plasmid

#125789

Purpose

constitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A (exonuclease-dead)

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN E84A K577M

Plasmid

#125791

Purpose

constitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A/K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A/K577M (exonuclease/helicase-dead)

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgMLH1-1

Plasmid

#125776

Purpose

constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgMLH1-1 (MLH1 Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgMLH1-2

Plasmid

#125777

Purpose

constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgMLH1-2 (MLH1 Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgWRN-EIJ

Plasmid

#125774

Purpose

constitutive expression of a guide RNA targeting an exon-intron junction of human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN-EIJ (WRN Human)

Use

CRISPR

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 581 - 600 of 1182 results

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