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pCMV-DNAJC5-ΔJ (Δ14-82)

Plasmid

#205719

Purpose

Mammalian expression of DNAJC5-ΔJ (Δ14-82)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

Δ14-82

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-L115R

Plasmid

#205708

Purpose

Mammalian expression of DNAJC5 L115R

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

L115R

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-L116Δ

Plasmid

#205709

Purpose

Mammalian expression of DNAJC5 L116Δ

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

L116Δ

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_V222A RAD23B

Plasmid

#201580

Purpose

Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation V222A

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_F400A RAD23B

Plasmid

#201581

Purpose

Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation F400A

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLI

Plasmid

#201440

Purpose

Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-Neo-ISG15

Plasmid

#80404

Purpose

overexpression of ISG15

Depositor

Isidoro Martinez

Article

Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

Insert

ISG15 (ISG15 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV3TAG8li-hRNF160-3FLAG

Plasmid

#159138

Purpose

human RNF160 (LTN1, ZNF294) FLAG mammalian expression vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

listerin E3 ubiquitin protein ligase 1 (LTN1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Tubulin-C-18

Plasmid

#231779

Purpose

Visualization of tubulin in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Tubulin-C-18 (TUBA1B Human)

Use

Tags

mGold2s

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Tubulin-C-18

Plasmid

#231771

Purpose

Visualization of tubulin in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Tubulin-C-18 (TUBA1B Human)

Use

Tags

mGold2s

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hADARB2-3xFLAG

Plasmid

#165470

Purpose

Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-eGFP-mPlum-HA

Plasmid

#200009

Purpose

Donor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence.

Depositor

Thomas Noll

Article

Hertel et al Front Bioeng Biotechnol. 2022 Oct 13;10:

Insert

eGFP, mPlum

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT RAD23A

Plasmid

#201458

Purpose

Expresses human RAD23A with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLH

Plasmid

#201671

Purpose

Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

Insert

Polymerase eta (POLH Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-GalT-mCherry

Plasmid

#214271

Purpose

Mammalian expression of GalT-mCherry (Golgi marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

GalT(1-82)-mCherry (B4GALT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMVd1-EGFP-hRhoA-wt

Plasmid

#133926

Purpose

Low level expression of GFP fused human RhoA wild type

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

GFP-hRhoA WT (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hMBOAT7-3xFLAG

Plasmid

#165474

Purpose

Human MBOAT7 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

Membrane bound O-acyltransferase domain containing 7 (MBOAT7 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RA-HA

Plasmid

#46859

Purpose

expresses mouse IL-17RA

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA (Il17ra Mouse)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-LAMP1-mCherry

Plasmid

#214274

Purpose

Mammalian expression of LAMP1-mCherry (lysosome marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

LAMP1-mCherry (Lamp1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV1-SMAC-HA-eGFP

Plasmid

#67487

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC (DIABLO Human)

Use

Tags

HA-eGFP

Expression

Mammalian

Mutation

aa1-59

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only