We narrowed to 1,842 results for: rigi

1. pUAS-hhN-HA

   Plasmid

   #240236

   Purpose

   Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

   Depositor

   Norbert Perrimon, Justin Bosch

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Insert

   hedgehog N-terminal fragment (hh Fly)
   

   Use

   Tags

   HA-spGFP11

   Expression

   Insect

   Mutation

   aa 1-257

   Promoter

   UAS

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. macroH2A2-GFP (pc2191)

   Plasmid

   #223709

   Purpose

   Expression of macroH2A2 tagged N-terminal to GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A2-GFP (MACROH2A2, H2AFY2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. macroH2A1.2-F192V-GFP (pc5115)

   Plasmid

   #223438

   Purpose

   Mutation phenylalanine 192 to valine (F192V) in macroH2A1.2-GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A1.2-F192V (Macroh2a1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Mutation of phenylalanine 192 to valine (F192V)

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW-ND-Cdt1Δ499-546-HA-Puro

   Plasmid

   #193768

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1Δ499-546 (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. macroH2A2-V192F-GFP (pc5116)

   Plasmid

   #223439

   Purpose

   Mutation of valine 192 to phenylalanine (V192F) in macroH2A2.

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A2-F192V (MACROH2A2, H2AFY2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Mutation of valine 192 to phenylalanine (V192F)

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

   Plasmid

   #193139

   Purpose

   Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

   Promoter

   EF1a

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pStABK1

   Plasmid

   #193729

   Purpose

   Start-Stop assembly level 1 vector, BBR1 origin, kanR

   Depositor

   Martin Buck

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. VfAU1-LOV_136

   Plasmid

   #63632

   Purpose

   VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a restriction site

   Depositor

   Harald Janovjak

   Insert

   VfAU1-LOV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pfNL43-dE-EGFP

   Plasmid

   #36865

   Depositor

   Mario Chin

   Insert

   fNL43-dE-EGFP
   

   Use

   Lentiviral and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   inserted EGFP after 6350 nucleotide of original N…

   Promoter

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLSU1/o35S[]:MCS:NosT

    Plasmid

    #212163

    Purpose

    This binary vector has an empty backbone where you can insert a gene into a cassette with a strong promoter (original 35sCAMV)

    Depositor

    Wayne Curtis

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRAC01A

    Plasmid

    #193730

    Purpose

    Expresses deactivated-Cas9 (J23101) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion

    Depositor

    Martin Buck

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRAC07A

    Plasmid

    #193733

    Purpose

    Expresses deactivated-Cas9 (J23107) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion

    Depositor

    Martin Buck

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. MYR-VfAU1-LOV_135

    Plasmid

    #63631

    Purpose

    Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a MYR signal and a restriction site

    Depositor

    Harald Janovjak

    Insert

    MYR-VfAU1-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-EcoR1-IRES-GFP

    Plasmid

    #66956

    Purpose

    original retroviral vector with one single EcoR1 cloning site

    Depositor

    Martine Roussel

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

15. P75ECD-VfAU1-LOV_448

    Plasmid

    #63633

    Purpose

    Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by the extracellular and transmembrane domain of p75NTR and a restriction site

    Depositor

    Harald Janovjak

    Insert

    P75ECD-VfAU1-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRPCN

    Plasmid

    #193726

    Purpose

    Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection

    Depositor

    Martin Buck

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pfNL43-dGPE-EGFP

    Plasmid

    #36866

    Depositor

    Mario Chin

    Insert

    NL43-dGPE-EGFP
    

    Use

    Lentiviral and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    parts of Gag and Pol were deleted and EGFP insert…

    Promoter

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRAC10A

    Plasmid

    #193734

    Purpose

    Expresses deactivated-Cas9 (J23110) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion

    Depositor

    Martin Buck

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. C1m

    Plasmid

    #120955

    Purpose

    MoClo golden gate assembly CD part for deGFP (deGFP3 with 4aa N-truncation, 9aa C-truncation, F64L, and C203T (numbered with original sequence); see https://www.fpbase.org/protein/degfp3/)

    Depositor

    Richard Murray

    Insert

    deGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOpen-RNAse H

    Plasmid

    #165575

    Purpose

    Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    RNAse H
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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