We narrowed to 9,253 results for: CAG

1. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201151

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   SadCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Mutation

   gRNA sequence: ATCAGTGATAGAGAACGTATG

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-hNoxa1

   Plasmid

   #58531

   Purpose

   expresses human Noxa1 in mammalian cells

   Depositor

   Karl-Heinz Krause, Botond Banfi

   Article

   Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

   Insert

   NADPH oxidase activator 1 (NOXA1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

   Plasmid

   #128749

   Purpose

   Expresses control gRNA

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-NEAT1pr_v2

   Plasmid

   #97082

   Purpose

   Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1pr_v2
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. miniCMV-iRFP670-tDeg

   Plasmid

   #185403

   Purpose

   iRFP670-tDeg fluorogenic protein

   Depositor

   Jian-Hui Jiang

   Article

   Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

   Insert

   iRFP670-tDeg
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet1-sgRNA(siteT9)

   Plasmid

   #154832

   Purpose

   sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Insert

   sgRNA(siteT9)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-blast shKGA

   Plasmid

   #110420

   Purpose

   shKGA, silence glutaminase KGA isoform, blasticidin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 (RNA Pol III)

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX-EGFP-g1

   Plasmid

   #107273

   Purpose

   eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Insert

   GFP sgRNA-1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiX2-PURO-shGFP

   Plasmid

   #61230

   Purpose

   Lentivirus expressing shRNA to GFP

   Depositor

   Reuben Shaw

   Article

   Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Promoter

   H1/TO

   Available Since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

10. PX458 pSp Cas9 Rhino1 tagA

    Plasmid

    #211627

    Purpose

    Cas9 Rhino1 tagA

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    RHNO tagA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTK73.ChrII_ttTi5605.sgRNA

    Plasmid

    #194058

    Purpose

    sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ChrII_ttTi5605 sgRNA
    

    Expression

    Worm

    Promoter

    U6

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCrx-DsRed

    Plasmid

    #13765

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Crx promoter (Crx Mouse)
    

    Tags

    DsRed2

    Expression

    Mammalian

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti‐puro‐OgRNA_mdxE53

    Plasmid

    #187054

    Purpose

    SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. ACE2 g2

    Plasmid

    #153012

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pELK3.1.0-gDNA

    Plasmid

    #112418

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3

    Depositor

    Kevin White

    Insert

    ELK3 (ELK3 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

    Plasmid

    #84254

    Purpose

    Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sa sgCXCR4-1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO-shTRAF2#1

    Plasmid

    #44127

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

18. H2B-GFP-AsLOV2-NES27 (pDN158)

    Plasmid

    #72659

    Purpose

    Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Insert

    H2B-GFP-AsLOV2-NES27
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. VPS35 shRNA1

    Plasmid

    #163858

    Purpose

    shRNA targeting the coding sequences of VPS35

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    VPS35 retromer complex component (VPS35 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAS9-mCherry-ACTG1

    Plasmid

    #66943

    Purpose

    CRISPaint target selector ACTG1

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA ACTG1
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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