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pcDNA3.2 mir-1-1 reporter dme-mir-34

Plasmid

#46665

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-34

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-1

Plasmid

#46651

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-1

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-lsy-6

Plasmid

#46650

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-lsy-6

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-lin-4

Plasmid

#46649

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-lin-4

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter cel-let-7

Plasmid

#46648

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-let-7

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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Khc73(1-387)-GFP

Plasmid

#31747

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Khc73 (Khc-73 Fly)

Tags

GFP

Expression

Mammalian

Mutation

Contains only aa 1-387

Promoter

Metallothionein

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only
Khc73(1-432)-GFP

Plasmid

#31748

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Khc73 (Khc-73 Fly)

Tags

GFP

Expression

Mammalian

Mutation

Contains only aa 1-432

Promoter

Metallothionein

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only
ptfLC3

Plasmid

#21074

Purpose

Mammalian expression of rat LC3 fused to mRFP and EGFP

Depositor

Tamotsu Yoshimori

Article

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP and mRFP

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCF806-shRen.713

Plasmid

#186712

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

shRen.713

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDOXOFF (+1)99xGly

Plasmid

#211347

Purpose

DOX-regulated lentiviral expression of +1 frameshifted 99xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

(+1)99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Mutation

+1 frameshift to produce polyR frame

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDOXOFF (+2)99xGly

Plasmid

#211348

Purpose

DOX-regulated lentiviral expression of +2 frameshifted 99xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

(+2)99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Mutation

+2 frameshift to produce polyA frame

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5R 99xGly

Plasmid

#211356

Purpose

Transient expression of 99xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

99xGly-EGFP-3xHA

Tags

EGFP-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5R 15xGly

Plasmid

#211355

Purpose

Transient expression of 15xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

15xGly-EGFP-3xHA

Tags

EGFP-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAM233

Plasmid

#226655

Purpose

pcDNA3.1-p97-FLAG

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCGN-HCF-1 fl

Plasmid

#53309

Purpose

plasmid contains human HCFC1 full length gene with HA and c-myc tags

Depositor

Winship Herr

Article

Wilson et al Cell. 1993 Jul 16;74(1):115-25.

Insert

HCFC1 (HCFC1 Human)

Tags

HA and c-myc

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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pCM184-ParB2::eGFP

Plasmid

#87252

Purpose

Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB2

Tags

EGFP

Expression

Yeast

Promoter

pBAD/tetO

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-OPA1

Plasmid

#26047

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

OPA1 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pET14b – E. coli Exonuclease I

Plasmid

#104552

Purpose

bacterial expression of Exonuclease I

Depositor

Charles Bell

Article

Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

Insert

Exonuclease I (sbcB E.coli)

Tags

6X-His tag followed by thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pmRFP-LC3

Plasmid

#21075

Purpose

Mammalian expression of rat LC3 fused to mRFP

Depositor

Tamotsu Yoshimori

Article

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

mRFP

Expression

Mammalian

Mutation

First ATG is not included.

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-col4a1

Plasmid

#160317

Purpose

cfSGFP2-tagged collagen4a1 for mammalian expression

Depositor

Ikuo Wada

Article

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Insert

COL4A1 (COL4A1 Human)

Tags

cfSGFP2

Expression

Mammalian

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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