161,152 results

1. pTRE3G-EBF1

   Plasmid

   #96965

   Purpose

   Dox-inducible expression of Ebf1 in mammalian cells

   Depositor

   Elena Cattaneo

   Article

   Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Insert

   Ebf1 (Ebf1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXS-GshF-blasticidin

   Plasmid

   #210313

   Purpose

   Expresses humanized Streptococcus thermophilus GshF in mammalian cells

   Depositor

   Kivanc Birsoy

   Article

   Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

   Insert

   Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
   

   Use

   Retroviral

   Tags

   FLAG peptide

   Expression

   Mutation

   Codon optimized for expression in human cells

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p23-NES-RfxCas13d-msfGFP-NES-Flag

   Plasmid

   #165076

   Purpose

   overexpression of RfxCas13d in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   RfxCas13d
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. phCMV-GALV-MTR

   Plasmid

   #163612

   Purpose

   Retro and Lentiviral transduction of primary human germinal center B cells

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Insert

   GaLV MTR envelope
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pemiRFP670-N1

   Plasmid

   #136556

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   emiRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-mhYFP

   Plasmid

   #186532

   Purpose

   Monomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Monomeric hyperfolder YFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   hfYFP-S147P/L195M/V206K

   Promoter

   araB

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRX-IB-pSu9-HaloTag7-mGFP

   Plasmid

   #184905

   Purpose

   Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   Presequence of Fo-ATPase subunit 9
   

   Use

   Tags

   HaloTag7 and monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lifeact-7-iRFP670

   Plasmid

   #103032

   Purpose

   Expression of iRFP670-tagged Lifeact with G418 selection in mammalian cells

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas3cRh

   Plasmid

   #133773

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Joseph Bondy-Denomy

   Article

   Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   Cas7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6.3>gRNA.f+e

    Plasmid

    #99139

    Purpose

    Empty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoter

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    chicken U6.3

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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