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pS448∙CsR

Plasmid

#122096

Purpose

CRISPR/Cas9 counterselection in Gram-negative bacteria

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Inserts

Cas9
sgRNA

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pem7 and Pm

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-Hyg

Plasmid

#210261

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMUH_unc84_2XGFP

Plasmid

#46023

Depositor

Sean Eddy

Article

Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

Insert

UNC-84 (unc-84 )

Use

10xuas-phi31-expression vector

Tags

2XGFP

Mutation

Two amino acid mutations (F279L and L500P) compar…

Promoter

10XUAS

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-3XNeon-nls

Plasmid

#75386

Purpose

PCP-3XNeon-nls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

PCP

Use

Lentiviral

Tags

3XmNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTH102

Plasmid

#84460

Purpose

pJB38-NWMN29-30 + SarA_P1-CFP-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-CFP from pTH2

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCN-EF2132tet

Plasmid

#107191

Purpose

Expresses EF2132 recombinase for performing recombineering in S. aureus

Depositor

Steve Salipante

Article

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

EF2132
tetR

Expression

Bacterial

Promoter

p23

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSLS.436

Plasmid

#184953

Purpose

Express -Eco1 RT and ncRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: ncRNA(wt) and RT

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ144

Plasmid

#69296

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-MCP-3XBFPnls

Plasmid

#75384

Purpose

MCP-3XBFPnls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

MCP

Use

Lentiviral

Tags

3XBFP

Expression

Mammalian

Promoter

EFS

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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gRNA Cloning Vector Bbs I ver. 2

Plasmid

#85586

Purpose

An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

Depositor

Hodaka Fujii

Article

Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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