We narrowed to 8,684 results for: App

1. pME iCre-I (JDW 1203)

   Plasmid

   #224520

   Purpose

   A Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteria

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   iCre-I
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-H2A_mCherry_SV40pA (JDW 967)

   Plasmid

   #224528

   Purpose

   A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   H2A-mCherry-SV40pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

   Plasmid

   #224532

   Purpose

   A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-H2A-mCherry-SV40pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3E EFS-rtTA/rtTA3 (JDW 1214)

   Plasmid

   #224533

   Purpose

   A Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivator

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   rtTA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)

   Plasmid

   #224534

   Purpose

   A Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tag

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-mNeonGreen-HA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3E-IRES-mTagBFP2-pA (JDW 1361)

   Plasmid

   #224535

   Purpose

   A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-FLAG-NLS-mTagBFP2-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-Lifeact- mScarlet-HA (JDW 1247)

   Plasmid

   #224538

   Purpose

   A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lifeact-mScarlet-HA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME H2B mCerulean (JDW 1150)

   Plasmid

   #224543

   Purpose

   A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   H2B-mCerulean
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5E 5X C120 c-fos min pro (JDW 1241)

   Plasmid

   #224544

   Purpose

   A Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   5x-C120-c-Fos promoter
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-MYC-As-NF-kB3

    Plasmid

    #180205

    Purpose

    Expresses MYC-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cells

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    As-NF-kB3
    

    Use

    Tags

    MYC-tagged fusion protein

    Expression

    Mammalian

    Mutation

    aa 2-412

    Promoter

    CMV

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. FGF8-P2A-iCre

    Plasmid

    #210800

    Purpose

    Recombinase reporter for FGF8 expression

    Depositor

    Yuejun Chen

    Article

    You et al Cell Stem Cell. 2023 Apr 6;30(4):473-487

    Insert

    FGF8 (FGF8 Human)
    

    Use

    Cre/Lox

    Tags

    iCre

    Expression

    Mammalian

    Mutation

    Promoter

    endougeneous FGF8 promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. APCDD1-P2A-tdTomato

    Plasmid

    #210801

    Purpose

    Fluorescent reporter for APCDD1 expression

    Depositor

    Yuejun Chen

    Article

    You et al Cell Stem Cell. 2023 Apr 6;30(4):473-487

    Insert

    APCDD1 (APCDD1 Human)
    

    Use

    Tags

    tdtomato

    Expression

    Mammalian

    Mutation

    Promoter

    endougeneous APCDD1 promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. T40A+Q54A

    Plasmid

    #217913

    Purpose

    A plasmid encoding the hydrogel-forming protein PXP with both the T40A and Q54A coil mutations

    Depositor

    Cole DeForest

    Article

    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

    Insert

    PXP T40A+Q54A
    

    Use

    Tags

    6x His (N and C term)

    Expression

    Bacterial

    Mutation

    Threonine at position 41 and 253 mutated to Alani…

    Promoter

    T5

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-FLAG-As-NF-kB2

    Plasmid

    #180201

    Purpose

    Expresses FLAG-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    As-NF-kB2
    

    Use

    Tags

    FLAG-tagged fusion protein

    Expression

    Mammalian

    Mutation

    aa 2-502

    Promoter

    CMV

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pC3.1_CMV_oROS-G_LF(C199S)

    Plasmid

    #216112

    Purpose

    Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G_LF(C199S)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

    Plasmid

    #217509

    Purpose

    KD Recombinase dependent conditional 1X ALFA cassette

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
    

    Use

    Tags

    ALFA tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV2_CAG_oROS-G_LF(C199S)

    Plasmid

    #216116

    Purpose

    Encodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G_LF(C199S)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. OptoProfilin.S138E

    Plasmid

    #208287

    Purpose

    Expresses Cry2(1-531)-mCherry-Profilin.S138E

    Depositor

    Robert Hughes

    Article

    Mann et al Chembiochem. 2024 May 2;25(9):e202400007

    Insert

    Cry2 - mCherry - Profilin.S138E (Cry2, PRF1 Mouse, Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ser 138 (sometimes numbered 137) mutated to Glu

    Promoter

    CMV

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. OptoProfilin.S138A

    Plasmid

    #208288

    Purpose

    Expresses Cry2(1-531)-mCherry-Profilin.S138A

    Depositor

    Robert Hughes

    Article

    Mann et al Chembiochem. 2024 May 2;25(9):e202400007

    Insert

    Cry2 - mCherry - Profilin (Cry2, PRF1 Mouse, Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ser 138 (sometimes numbered 137) mutated to Ala

    Promoter

    CMV

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15

    Plasmid

    #207054

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    L55W T56A V61W R65A

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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