We narrowed to 8,353 results for: Set;

1. pCfB8382

   Plasmid

   #126916

   Purpose

   Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtI
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBT374_pattB-pCA-GFP-pA-attBSwa

   Plasmid

   #52553

   Purpose

   for PhiC31 integrase-mediated cassette exchange

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attB (full length)
   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDDIT3-donor

   Plasmid

   #112300

   Purpose

   CRISPR donor plasmid to tag human transcription factor DDIT3 with GFP

   Depositor

   Kevin White

   Insert

   DDIT3 homology arms flanking EGFP-IRES-Neo cassette (DDIT3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. GGE254

   Plasmid

   #120789

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_Lip-NotI
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GGE255

   Plasmid

   #120788

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_Lip-NotI
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTV-Fbx15

   Plasmid

   #13464

   Depositor

   Shinya Yamanaka

   Article

   Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

   Insert

   IRES-Bgeo-pA cassette flanked by Fbx15 homologous arms
   

   Use

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pHOXB5-donor

   Plasmid

   #113815

   Purpose

   CRISPR donor plasmid to tag human transcription factor HOXB5 with GFP

   Depositor

   Kevin White

   Insert

   HOXB5 homology arms flanking EGFP-IRES-Neo cassette (HOXB5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   rs2288276, rs7406798, rs36049616, rs3087996

   Promoter

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

   Plasmid

   #162810

   Purpose

   STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   BsmBI_Intein_DD
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGPS21loxFRTNeo

   Plasmid

   #27180

   Depositor

   Yoh Wada

   Article

   Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

   Insert

   lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R
   

   Use

   In vitro transposion

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pOVC1_L3(GSSSSGGSSSG)

    Plasmid

    #131210

    Purpose

    Cloning vector containing A-B-C casette with linker L3 in site B

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    GSSSSGGSSSG llinker
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOVC1_L2(GSSGGSSG)

    Plasmid

    #131209

    Purpose

    Cloning vector containing A-B-C casette with linker L2 in site B

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    GSSGGSSG linker
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOVC1_L1(GSSSG)

    Plasmid

    #131208

    Purpose

    Cloning vector containing A-B-C casette with linker L1 in site B

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    GSSSG linker
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAEF6

    Plasmid

    #136303

    Purpose

    pAEF5 plasmid with two gRNA inserted

    Depositor

    Gilles Fischer

    Article

    Agier et al Methods Mol Biol. 2021;2196:181-198. doi

    Insert

    double cassette coding for gRNA
    

    Use

    CRISPR; Template for pcr to insert double grna co…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCLOCK-donor

    Plasmid

    #112310

    Purpose

    CRISPR donor plasmid to tag human transcription factor CLOCK with GFP

    Depositor

    Kevin White

    Insert

    CLOCK homology arms flanking EGFP-IRES-Neo cassette (CLOCK Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:4:55435551:A>G, hg38:4:55435749:G>A

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRC02

    Plasmid

    #114681

    Purpose

    sfGFP.Strand10-Haloenzyme

    Depositor

    Matthew Good

    Article

    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

    Insert

    sfGFP.Strand10-Haloenzyme
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. KG#230

    Plasmid

    #110925

    Purpose

    Vector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cord

    Depositor

    Kenneth Miller

    Article

    Edwards et al J Cell Biol. 2009 Sep 21;186(6):881-95.

    Inserts

    unc-129 promoter
    unc-54 3' control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. BASIC_10_J23101-RBS32-GFP-B0015

    Plasmid

    #68144

    Purpose

    storage vector for BASIC part: GFP expression cassette

    Depositor

    Geoffrey Baldwin

    Article

    Storch et al ACS Synth Biol. 2015 Apr 16.

    Insert

    J23101-RBS32-GFP-B0015
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFLIP30

    Plasmid

    #65499

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-mCer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAW8ENdeI-cyEGFP-spo5DSR

    Plasmid

    #49867

    Purpose

    for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    yEGFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFLIP32

    Plasmid

    #65500

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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