We narrowed to 18,916 results for: Tec

1. pGWB423

   Plasmid

   #74817

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-RapR-FAK-YM

   Plasmid

   #25928

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

   Insert

   RapR-FAK (Ptk2 Mouse, Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   This pEGFP-RapR-FAK-YM plasmid contains the FAK m…

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOCC291

   Plasmid

   #118867

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease, mRuby3

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pWL89A mmPing20

   Plasmid

   #145788

   Purpose

   mPing Yeast Transposition Assay

   Depositor

   Charles Nathan Hancock

   Article

   Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

   Insert

   mmPing20
   

   Expression

   Yeast

   Mutation

   T162C, T258A, T287C, T300A, T304A, T310A, G372A

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCellFree_G03 CALR

   Plasmid

   #67086

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CALR (CALR Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-dCpf1-eBE-YE

   Plasmid

   #107689

   Purpose

   Expresses dLbCpf1-eBE-YE in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-eBE-YE
   

   Expression

   Mammalian

   Mutation

   APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

   Promoter

   CMV

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CAG-CreiLOV-P2A-FusionRed

   Plasmid

   #192818

   Purpose

   Expresses CreiLOV with FusionRed tag in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   CreiLOV
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJL1-MBP-4xDQNAT

   Plasmid

   #128390

   Purpose

   In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Maltose binding protein
   

   Tags

   4xDQNAT glycosylation tag and 6xHis tag

   Expression

   Bacterial

   Mutation

   A302T- please see depositor comment

   Promoter

   T7

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eNme2-C TadCBEa

   Plasmid

   #193840

   Purpose

   Express TadCBEa (with eNme2-C variant) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   eNme2-C TadCBEa
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBEAST-pBen-Luc

    Plasmid

    #122694

    Purpose

    Output expression of firefly luciferase under the activation of BenR transcription factor for cell-free expression

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    Firefly luciferase
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21b-rrt-His8

    Plasmid

    #194088

    Purpose

    Expresses Ec86 reverse transcriptase in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 rrt
    

    Tags

    His8

    Promoter

    T7

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTorPE-IP2.0

    Plasmid

    #109217

    Purpose

    Express IP2.0 in E. coli

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p2CT-His-MBP-Lbu_C2c2_R1079A

    Plasmid

    #83494

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R1079A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R1079A

    Promoter

    T7

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOCC72

    Plasmid

    #118866

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, mGFP, cleavable…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CEBPalpha NGFR

    Plasmid

    #44627

    Depositor

    Ellen Rothenberg

    Article

    Del Real et al Development. 2013 Mar;140(6):1207-19. do

    Insert

    CEBPalpha (Cebpa Mouse)
    

    Use

    Retroviral

    Tags

    IRES and truncated NGFR

    Expression

    Mammalian

    Available Since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCS2-Flag-TTGZFP-FokI-DD

    Plasmid

    #18755

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    zinc finger nuclease
    

    Expression

    Bacterial

    Mutation

    RNA expression vector for expressing the zinc fin…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEL030

    Plasmid

    #137899

    Purpose

    STU2.0 SpyCas9 Csy4 system for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSI-359

    Plasmid

    #131131

    Purpose

    gRNA expression vector for A-to-G base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    A-to-G reporter activation gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L2-AA007

    Plasmid

    #185750

    Purpose

    Vector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Inserts

    HygR2
    eGFP
    

    Tags

    membrane localization tag Lti6b

    Expression

    Plant

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNXgate32-3HA

    Plasmid

    #105076

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Tags

    3xHA and NubG

    Expression

    Yeast

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge