159,557 results

1. pNP1047

   Plasmid

   #222726

   Purpose

   pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 bridge RNA
   

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

   Plasmid

   #175573

   Purpose

   Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. YPet-N1

   Plasmid

   #54637

   Purpose

   Localization: N1 Cloning Vector, Excitation: 513, Emission: 527

   Depositor

   Patrick Daugherty, Michael Davidson

   Article

   Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

   Type

   Empty backbone

   Use

   Tags

   YPet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. KAT14-pDEST10

   Plasmid

   #118382

   Purpose

   HIS-tagged insect cell expression vector with KAT14.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   KAT14 (KAT14 Human)
   

   Use

   Tags

   HIS

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti CMVtight eGFP Blast (w782-1)

   Plasmid

   #26584

   Purpose

   2nd generation lentiviral vector

   Depositor

   Eric Campeau

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Lentiviral; Tet-on advanced vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-Flag-Tet3

   Plasmid

   #60940

   Purpose

   Expresses Tet3 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Ten-Eleven Translocation 3
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX-RCC1-HA

   Plasmid

   #106938

   Purpose

   Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

   Depositor

   Patrizia Lavia

   Article

   DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

   Insert

   human RCC1-HA (RCC1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-GAL4-UAS-huCD25- IRES-mCherry-PGK-BFP

   Plasmid

   #223211

   Purpose

   Lentiviral vector - mCherry reporter and huCD25 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   CD25 (IL2RA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRC_RR_VP1_r1c3

   Plasmid

   #65724

   Purpose

   Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR

   Depositor

   Junghae Suh

   Article

   Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMDG

    Plasmid

    #187440

    Purpose

    VSV-G envelope expressing plasmid

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Inserts

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLG327

    Plasmid

    #128797

    Purpose

    Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    gfpmut2 (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pxyl(s)

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. GfaABC1D-jGCaMP8m

    Plasmid

    #213010

    Purpose

    To study calcium signaling in Astrocytes

    Depositor

    Baljit Khakh

    Article

    Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

    Insert

    GfaABC1D-jGCaMP8m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b-SpG-His

    Plasmid

    #179318

    Purpose

    Plasmid for bacterial expression and purification of SpG-Cas9

    Depositor

    Julián Cerón, Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpG
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. G3BP1-Grx1-roGFP2

    Plasmid

    #236548

    Purpose

    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in stress granules.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    G3BP1-Grx1-roGFP2 (G3BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYESir2-puro

    Plasmid

    #1769

    Depositor

    Bob Weinberg

    Article

    Vaziri et al Cell 2001 Oct 19;107(2):149-59.

    Insert

    Sir2 (SIRT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRE-Tn7-165

    Plasmid

    #118577

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAS_sfGPF 150TAG

    Plasmid

    #154765

    Purpose

    plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH_Str-KDEL_ST-SBP-EGFP

    Plasmid

    #65267

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. mApple-Sec61b-C1

    Plasmid

    #90993

    Purpose

    Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mApple-Sec61b (SEC61B Synthetic, Human)
    

    Use

    Tags

    mApple (red fluorescence)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. CNOT3

    Plasmid

    #155467

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CNOT3 (CNOT3 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge