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Showing: 6021 - 6040 of 176472 results

  2. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Publication
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    163148-plasmid-map-sequence-id-331409

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  4. pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

    Plasmid
    #162279
    Purpose
    Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Publication
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN2A Variant 1, stabilized (SCN2A Human)
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp4551.
    Promoter
    CMV
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    162279-plasmid-map-sequence-id-321873

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  16. pGP-AAV-syn-jGCaMP7s-WPRE

    Plasmid
    #104487
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has service
    AAV1, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    jGCaMP7s
    Use
    AAV
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42 Articles
    104487-plasmid-map-sequence-id-241275

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  19. pET28a-AviTag-SnoopLigase

    Plasmid
    #105626
    Purpose
    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation
    Depositor
    Mark Howarth
    Publication
    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3
    Insert
    AviTag-SnoopLigase
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    105626-plasmid-map-sequence-id-205296

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  20. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    100902-plasmid-map-sequence-id-195323

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Showing: 6021 - 6040 of 176472 results