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pET28b-miniE-DBCO

Plasmid

#181985

Purpose

Used to label proteins with the MiniR genetically encoded tag

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

MiniE-DBCO

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-FGD1

Plasmid

#129612

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

FGD1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-E. coli Initiation Factor 3 (IF3)

Plasmid

#31340

Depositor

Linda Spremulli

Article

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

E. coli Initiation Factor 3

Tags

His

Expression

Bacterial

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pME-tdTomato no stop

Plasmid

#82404

Purpose

Gateway-compatible tdTomato fusion reporter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

tdTomato (lacks stop codon)

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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JGE302_pAAV-hSyn-DIO-Myc-PYLcs-bArrestin2-YFP (p2)

Plasmid

#107247

Purpose

beta arrestin 2 fused to PYLcs for abscisic acid mediated recruitment of ABIcs labelled proteins.

Depositor

Bryan Roth

Article

Roth et al bioRxiv 251769

Insert

ARRB2

Use

AAV

Tags

MYC, PYLcs, and YFP

Promoter

hSYN

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestGFP_HuD

Plasmid

#65763

Purpose

expresses GFP-tagged HuD in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5-BMLF1

Plasmid

#72636

Purpose

Mammalian expression vector for Epstein-Barr virus Mta immediate-early post-transcriptional regulator

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BMLF1/BSLF2

Expression

Mammalian

Mutation

Q157H

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pKSJ331

Plasmid

#103063

Purpose

Expresses colicin E1 and E1 immunity protein without E1 lysis (kil) gene

Depositor

Alan Finkelstein, Karen Jakes

Article

Kienker et al J Gen Physiol. 2008 Dec;132(6):693-707.

Insert

Colicin E1 and E1 immunity protein

Expression

Bacterial

Promoter

colicin promotor from ColE1

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1-v5.1+T1-empty

Plasmid

#204977

Purpose

AsCas12f1-v5.1+T1-based Human cell genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nature Catalysis (2023) 6, 695–709

Type

Empty backbone

Use

CRISPR

Mutation

N70Q/K103R/A104R/S118A/D364R

Promoter

PCMV,U6

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV Aquamarine

Plasmid

#157777

Purpose

Expresses cyan fluorescent Aquamarine in cells

Depositor

Marc Tramier

Article

Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pstb-LAFR5

Plasmid

#86169

Purpose

pLAFR5 with Smb21651 promoter. pLAFR5 is a broad-host range cosmid vector with double cos sites.

Depositor

Kay Jones

Article

Jones et al J Bacteriol. 2012 Aug;194(16):4322-31. d

Type

Empty backbone

Expression

Bacterial

Promoter

SMb21651 promoter

Available Since

Feb. 6, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pET 15b-UbcH5a/2417

Plasmid

#11472

Depositor

Allan Weissman

Article

Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

Insert

UbcH5a (UBE2D1 Human)

Expression

Bacterial

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
EMM67

Plasmid

#49043

Purpose

Backbone contains SHD/G 0.5 Domain TALE N-terminal TALE C-terminus, LSD1: Isoform 2

Depositor

Bradley Bernstein, Eric Mendenhall

Article

Mendenhall et al Nat Biotechnol. 2013 Sep 8. doi: 10.1038

Type

Empty backbone

Use

Tale lsd1 expression vector

Tags

LSD1

Expression

Mammalian

Promoter

EF1 alpha

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pBY2982

Plasmid

#23220

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

BirA-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pTHSSd_41

Plasmid

#59964

Purpose

High full length T7 RNAP* expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T7* RNAP

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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EMM65

Plasmid

#49042

Purpose

Backbone contains SHD/C 0.5 Domain TALE N-terminal: TAL C-terminus: LSD1: Isoform 2

Depositor

Bradley Bernstein, Eric Mendenhall

Article

Mendenhall et al Nat Biotechnol. 2013 Sep 8. doi: 10.1038

Type

Empty backbone

Use

Tale lsd1 expression vector

Tags

LSD1

Expression

Mammalian

Promoter

EF1 alpha

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pKM263-ScPPX

Plasmid

#38327

Depositor

Florian Freimoser

Article

Werner et al Arch Microbiol. 2005 Nov;184(2):129-36.

Insert

PPX1 (PPX1 Budding Yeast)

Tags

GST, His, and TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestRFP_HuD

Plasmid

#65766

Purpose

expresses RFP-tagged HuD in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Expression

Mammalian

Mutation

V23M mutation in RFP

Promoter

CMV, 2x Tet

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
CAG-NeuroD2iresGFP

Plasmid

#45026

Depositor

Connie Cepko

Article

Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

Insert

NeuroD2 (Neurod2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG tubulin GFP

Plasmid

#66105

Purpose

expression of chicken tubulin GFP

Depositor

Felicitas Proels, Martin Scaal

Article

et al Development. 2015 Feb 15;142(4):665-71.

Insert

tubulin

Use

Chicken expression

Tags

EGFP

Promoter

CAG

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only