We narrowed to 8,601 results for: Set

1. OCT4-2A-mOrange

   Plasmid

   #66986

   Purpose

   Generate OCT4 mOrange reporter line without selection cassette

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

   Insert

   2A-mOrange
   

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRC319

   Plasmid

   #61272

   Purpose

   Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGD289

   Plasmid

   #228113

   Purpose

   pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Kva1 retron, ncRNA with 90 bp rpoB donor
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB8787

   Plasmid

   #126921

   Purpose

   Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   HMG1
   

   Expression

   Yeast

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSkipFlox

   Plasmid

   #85797

   Purpose

   P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

   Depositor

   Tobias Spielmann

   Article

   Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   split Cre recombinase
   

   Use

   Cre/Lox; P. falciparum expression

   Tags

   FKBP, FRB*

   Promoter

   Pfcrt

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEED-Dendra2

   Plasmid

   #219645

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Dendra2 SEED Cassette
   

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVKG1-Puro

   Plasmid

   #108670

   Purpose

   Puromycin resistant cassette for knock-in by VIKING method

   Depositor

   Shigeo Sugano

   Article

   Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

   Insert

   PURO
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEED-OLLAS:HA

   Plasmid

   #219647

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   OLLAS:HA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB8748

   Plasmid

   #126918

   Purpose

   Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtE
   

   Expression

   Yeast

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKAd5f11pES-PmeI

    Plasmid

    #122698

    Purpose

    To generate an E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and the exogenous gene expression cassette.

    Depositor

    Zhuo-Zhuang Lu

    Article

    Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

    Type

    Empty backbone

    Use

    Adenoviral

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB8788

    Plasmid

    #126922

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and HMG1
    

    Expression

    Yeast

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-LRF

    Plasmid

    #66936

    Purpose

    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

    Depositor

    Alvaro Monteiro

    Article

    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV-40

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

13. PB-UBC-mARG3

    Plasmid

    #173793

    Purpose

    Mammalian acoustic reporter gene 1 cassette 3 on PB-UBC plasmid

    Depositor

    Xiaojun Lian

    Article

    Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

    Insert

    GvpJ, F, G, L, K
    

    Expression

    Mammalian

    Promoter

    UBC promoter

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179027

    Purpose

    Parent vector for markerless yeast integration cassettes with untagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. ptRNAfMet-A37

    Plasmid

    #196657

    Purpose

    Plasmid for overexpression of native initiator tRNA (A37)

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

    Insert

    tRNAfMet-A37 expression cassette
    

    Expression

    Bacterial

    Promoter

    metY native promoter

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-FlEx switch

    Plasmid

    #82406

    Purpose

    Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Type

    Empty backbone

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgDonor_lentiGuide

    Plasmid

    #184168

    Purpose

    LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Insert

    sgDonor
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAH25-SceI

    Plasmid

    #129389

    Purpose

    Derived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Chloramphenicol acetyltransferase
    

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    CAT promoter

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAD6

    Plasmid

    #134349

    Purpose

    E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

    Depositor

    Seth Darst

    Article

    Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    mfd (mfd E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB8786

    Plasmid

    #126920

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Expression

    Yeast

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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