We narrowed to 7,295 results for: ust

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TurboID-MEK1

Plasmid

#226169

Purpose

Used to transiently express a TurboID fusion of the protein kinase MEK1.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-TurboID-MEK1 (MAP2K1 Human)

Tags

Myc-TurboID

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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ShadowY-CLC

Plasmid

#193560

Purpose

Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

ShadowY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CLC-ShadowY

Plasmid

#193561

Purpose

Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

ShadowY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FluoSTEP-AKAR (C/Y)

Plasmid

#181862

Purpose

FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AKAR (C-Y)

Tags

CFP(1-10) and cpVenus(E127)

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-H2BC11_sgRNA

Plasmid

#183883

Purpose

pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-H2BC11_sgRNA

Plasmid

#183881

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pJJGL003

Plasmid

#180607

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

DpbCasX with R3 loop

Use

CRISPR

Tags

10x His and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xQUAS-EBFP2-GMRWhite

Plasmid

#165921

Purpose

Blasticidin resistant 5xQUAS EBFP2 GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xQUAS-sfGFP-MiniWhite

Plasmid

#165922

Purpose

Blasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xQUAS-EBFP2-MiniWhite

Plasmid

#165924

Purpose

Blasticidin resistant 5xQUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xUAS-EBFP2-MiniWhite

Plasmid

#165912

Purpose

Blasticidin resistant 5xUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable UAS Response Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pG418R-MCS-LexA-VP16-MiniWhite

Plasmid

#165894

Purpose

G418 resistant LexA driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xUAS-mCherry-GMRWhite

Plasmid

#165908

Purpose

Blasticidin resistant 5xUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable UAS Response Vector

Use

Synthetic Biology

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xQUAS-mCherry-GMRWhite

Plasmid

#165920

Purpose

Blasticidin resistant 5xQUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-12xLexAOp-sfGFP-MiniWhite

Plasmid

#165916

Purpose

Blasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xQUAS-mCherry-MiniWhite

Plasmid

#165923

Purpose

Blasticidin resistant 5xQUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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KG#797

Plasmid

#110916

Purpose

Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

Inserts

unc-129 promoter
STRN-1 (Sentryn)-GFP
unc-54 3' control region with 1 intron just upstream and 1 intron in control region

Tags

GFP (contains 3 artificial introns)

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/OCTN2 (delta554_557/PDZ)

Plasmid

#112803

Purpose

Expression of OCTN2 mutant

Depositor

Katarzyna Nalęcz

Article

Jurkiewicz et al Biochim Biophys Acta. 2017 May;1864(5):7

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Tags

3 x Flag

Expression

Mammalian

Mutation

amino acids 554_557 deleted/PDZ

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/OCTN2 (delta14_22)

Plasmid

#112800

Purpose

Expression of OCTN2 mutant

Depositor

Katarzyna Nalęcz

Article

Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Tags

3 x Flag

Expression

Mammalian

Mutation

amino acids 14_22 deleted

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 P294A

Plasmid

#112102

Purpose

Mammalian expression of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

P294A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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