164,802 results

1. pJR98

   Plasmid

   #187239

   Purpose

   CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAM1

   Plasmid

   #60487

   Purpose

   This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHel3

   Plasmid

   #102961

   Purpose

   E.coli/H.Pylori shuttle vector

   Depositor

   Rainer Haas

   Article

   Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

   Type

   Empty backbone

   Use

   Shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. eGFP-SUN1

   Plasmid

   #213515

   Purpose

   Expresses human eGFP-SUN1 in mammalian cells

   Depositor

   Karim Mekhail

   Article

   Shokrollahi et al bioRxiv 2023.05.07.539750

   Insert

   SUN1 (SUN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SUN1 has a silent mutation on its gene (nucleotid…

   Promoter

   CMV

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas9-DNMT3A-EGFP

   Plasmid

   #71666

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPLICS PM-ER Long P2A

   Plasmid

   #164111

   Purpose

   Detect the long Plasma membrane-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP PM-ER Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNCS mCherry

   Plasmid

   #91769

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Cherry
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   See Depositor Comments

   Promoter

   T7

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-Pur-CAG-hM3Dq-mCherry

   Plasmid

   #80948

   Purpose

   human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   hM3Dq-mCherry
   

   Use

   Tags

   mcherry fusion

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP_TRPM8

   Plasmid

   #64879

   Purpose

   Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

   Depositor

   Sebastián Brauchi

   Article

   Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

   Insert

   Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)
   

   Use

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-Ftractin-mCherry

    Plasmid

    #85131

    Purpose

    lentiviral expression of an F-actin live-cell marker (mCherry)

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC0046-EF1a-PspCas13b-NES-HIV

    Plasmid

    #103862

    Purpose

    Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pNF02-mLemon

    Plasmid

    #219399

    Purpose

    Constitutive expression of mLemon in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mLemon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    proDp

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP

    Plasmid

    #119903

    Purpose

    Bxb1 integration plasmid for cuO array with Puro selection cassette

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    cuOx144 array
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mNG2(1-10)_tdTomato

    Plasmid

    #184169

    Purpose

    Lentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(1-10)
    tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV2/7

    Plasmid

    #112863

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap7
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Truncated P5

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 acGFP

    Plasmid

    #128047

    Purpose

    Mammalian expression vector with N terminal AcGFP

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-pHluorin_M153R-CD63

    Plasmid

    #172117

    Purpose

    Fluorescent reporter for CD63-positive exosome secretion

    Depositor

    Alissa Weaver

    Article

    Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

    Insert

    CD63 (CD63 Human)
    

    Use

    Lentiviral

    Tags

    pHluorin_M153R in a small extracelluar loop of CD…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1-Pur-CAG-hM4Di-mCherry

    Plasmid

    #80947

    Purpose

    human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    hM4Di-mCherry
    

    Use

    Tags

    mcherry fusion

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIS1-Eef25UTR-renilla

    Plasmid

    #38235

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Eef2 5'UTR / Renilla (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Eef2 5' UTR fused to renilla ORF

    Promoter

    Endogenous Eef2

    Available Since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-HA-ER Y537S

    Plasmid

    #49499

    Purpose

    Expresses HA-tagged ER Y537S mutant in mammalian cells

    Depositor

    Sarat Chandarlapaty

    Article

    Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Tyrosine 537 to Serine

    Promoter

    CMV

    Available Since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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