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pMRE-Tn7-161

Plasmid

#118573

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG PSAM4 GlyR IRES EGFP

Plasmid

#119739

Purpose

Chemogenetic inhibitor expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

CAG

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-MT GLI2 delta N

Plasmid

#17649

Depositor

Erich Roessler

Article

Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

Insert

GLI2 (GLI2 Human)

Tags

6x Myc

Expression

Mammalian

Mutation

Missing 328 N-terminal amino acids

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCas3-Km

Plasmid

#207999

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-mGFP

Plasmid

#62629

Purpose

mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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Emerin pEGFP-C1 (637)

Plasmid

#61993

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

Emerin (EMD Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBV-Luc wt MBS1-4

Plasmid

#16564

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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pEVOL-AckRS

Plasmid

#137976

Purpose

sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

Depositor

Wenshe Liu

Article

Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

Insert

AckRS and pylTcua

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-152

Plasmid

#118534

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-142

Plasmid

#118558

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-147

Plasmid

#118563

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-ASF1A

Plasmid

#31591

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFPc3 N-RAS

Plasmid

#236450

Purpose

transient overexpression of N-RAS in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

N-RAS (NRAS Human)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(mem).iGlucoSnFR2

Plasmid

#244081

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pQTEV-COPE

Plasmid

#31585

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

coatomer protein complex, subunit epsilon (COPE Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCas3-Sm

Plasmid

#208001

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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AAT-PB-CG2APtk

Plasmid

#86003

Purpose

homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

eGFP_T2A_puro_dTK

Use

Piggybac exciseable

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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p221a-GUS

Plasmid

#71263

Purpose

Entry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

Beta-glucuronidase

Use

Gateway

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP8

Plasmid

#42311

Depositor

Thierry Galli

Article

Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

Insert

VAMP 8 (VAMP8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR221-mRuby3

Plasmid

#166136

Purpose

Gateway entry vector for mRuby3

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

mRuby3

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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