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Plasmid#124307PurposeLentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoterDepositorInsertNedd8 (NEDD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationFive C-terminal amino acids missing compared to w…PromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry
Plasmid#176277PurposeViral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.DepositorInsertMCS-P2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension
Plasmid#176542PurposeBacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locusDepositorInsertsMBP tag
TnpB
UseTags3' extension derived from endogenous locus a…ExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYFP-ErbB2
Plasmid#66948PurposeMammalian expression of rat ErbB2 tagged with YFPDepositorInsertErbB2 (Erbb2 Rat)
UseTags6xHis, V5 Tag, and YFPExpressionMammalianMutation(See depositor comment below on mutations A24T an…PromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW1,2,4
Plasmid#171137Purposeexpresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cellsDepositorInsertNEDD4L (NEDD4L Human)
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 221 to alanine, tryptophan 453…PromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_OTmut
Plasmid#185389PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin control sensor GRAB_OTmut
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-hGH
Plasmid#182411PurposeBacterial expression of Vesicle Nucleating peptide-human growth hormone fusionDepositorInsertVNp-hGH (GH1 Human)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpIMPDH
Plasmid#206980PurposeStreptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.DepositorInsertSpIMPDH (guaB Streptococcus pyogenes)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
PITX3-eGFP-PGK-Puro
Plasmid#31942DepositorInsertPITX3 (PITX3 Human)
UseTargeting donorTagseGFPExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A T31A-dCas9
Plasmid#163537PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-dCas9
UseCRISPRTagsExpressionMammalianMutationdCas9 (D10A, H840A)eA3A T31APromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc human MST2
Plasmid#37022DepositorInsertMST2 (STK3 Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVH-EF1a-GFP-P2A-SOX2
Plasmid#130693PurposeLentiviral plasmids encoding human SOX2 with co-expressoin of GFP mediated by P2A.DepositorInserthuman SOX2 (SOX2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
TV-hPITX3-forward
Plasmid#22073DepositorInsertPITX3 (ASMD Human)
UseCre/Lox; Zfn donorTagseGFPExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-nCas9
Plasmid#163546PurposeMammalian CG-to-GC base editingDepositorInsertRBMX-eA3A-UdgX-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-hCYP26A1-FL- luciferase
Plasmid#135566PurposeFull Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferaseDepositorInsertFull-length promoter of human retinoic acid receptor-beta gene
UseTagsExpressionMutationPromoterhHuman CYP26A1 gene promoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTER E2F1shRNA human
Plasmid#66883PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F1DepositorInsertE2F transcription factor 1 (E2F1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDR366 RNF2
Plasmid#47509Purposehuman gRNA expression vector targeting RNF2DepositorInsertgRNA-RNF2 (RNF2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-OS
Plasmid#136576PurposeDox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2DepositorUseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-CD81-(TEV)-FKBP-Myc
Plasmid#177908Purposehuman CD81-TEV cleavage site-FKBP-Myc tagDepositorInserthuman CD81 (CD81 Human, Synthetic)
UseTagsFKBP, Myc tag, and TEV protease cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only