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Showing: 6061 - 6080 of 20007 results

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p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

Plasmid

#227773

Purpose

Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

Depositor

Bushra Raj

Publication

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

U6 and tp1

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

Plasmid

#227774

Purpose

Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

Depositor

Bushra Raj

Publication

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

loxP-dsR-loxP

Use

CRISPR

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMT Draper-Ala11-GFP

Plasmid

#126654

Purpose

Inducible expression of Draper-Ala11-GFP mutant in S2 cells

Depositor

Ron Vale

Publication

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmi Tyr residues mutated to Ala

Expression

Insect

Mutation

Cytoplasmic Tyr residues to Ala

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMT Shark-TagBFP

Plasmid

#126658

Purpose

Inducible expression of Shark-TagBFP in S2 cells

Depositor

Ron Vale

Publication

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark

Expression

Insect

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-T7-OmpA-MNaseB-6xHis

Plasmid

#214809

Purpose

Expression of 6xhis-tagged micrococcal nuclease B (MNaseB)

Depositor

Peter Sarin

Publication

Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

Insert

OmpA-Micrococcal nuclease B

Tags

6xhis

Expression

Bacterial

Promoter

T7 promoter

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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T2A GFP H2B

Plasmid

#227722

Purpose

Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Publication

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

(nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)

Use

Cloning vector

Tags

T2A 3xNLS GFP

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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SL2 GFP H2B

Plasmid

#227717

Purpose

Plasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Publication

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SL2 GFP H2B (nlp-51 Nematode)

Use

Cloning vector

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SH2

Plasmid

#209704

Purpose

Mammalian expression of SH2

Depositor

Stewart Loh

Publication

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SH2

Expression

Mammalian

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pF hPGK mc2 IRES neo

Plasmid

#206236

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pF CAG mc2 IRES neo

Plasmid

#206232

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p5E_(-3kb)nrg1 type II

Plasmid

#199393

Purpose

pDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream regulatory sequence

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

(-3kb)nrg1 type II

Use

Gateway Cloning

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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p3E_afp_UTR

Plasmid

#199341

Purpose

pAMS448; Gateway 3' entry clone encoding ocean pout antifreeze (afp) protein 3'UTR

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

afp 3' UTR

Use

Gateway Cloning

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2_(-3kb)nrg1_typeII:GFP-CAAX

Plasmid

#199394

Purpose

pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR

Use

Tol2 gateway destination vector

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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vgCam-pRSETb

Plasmid

#193362

Purpose

A FRET-based calcium probe using Sumire

Depositor

Takeharu Nagai

Publication

Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

vgCam

Use

E.coli expression

Tags

His X6 tag and Strep tag

Promoter

T7

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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p8267 LentiCRISPR v2 hygro sgNT-2

Plasmid

#193978

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Publication

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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p8203 MSCV-neo C-V5 16E7

Plasmid

#193976

Purpose

HPV16 E7 expression, C-terminal V5 tag

Depositor

Elizabeth White

Publication

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

HPV16 E7

Use

Retroviral

Tags

V5

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-B-frSkyran-S

Plasmid

#182209

Purpose

Bacterial expression of frSkylan-S

Depositor

Shiro Suetsugu

Publication

Osuga et al Mol Biol Cell. 2021 Nov 1;32(21):br7. do

Insert

frSkylan-S

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK086

Plasmid

#171358

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

P-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK063

Plasmid

#171335

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

dSpCas9(m4) (for fusion)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK062

Plasmid

#171334

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

dSpCas9(m2) (for fusion)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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