164,223 results

1. SepOTSλ

   Plasmid

   #68292

   Purpose

   Phosphoserine orthogonal translation system (preferred variant)

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS9/EFSep21, 4xtRNA-Sep-A37
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-mTH-GFP

   Plasmid

   #99128

   Purpose

   An AAV genome that expresses GFP from the mouse TH promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mTH

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOSIP-KC (KanR, PhiC31)

   Plasmid

   #45982

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. His6-MBP-uTEV3

   Plasmid

   #135464

   Purpose

   Bacterial expression of uTEV3 (full-length) protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   His6-MBP-uTEV3
   

   Use

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation (improves stability) , I138T/S153N…

   Promoter

   T5

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV pCAG-FLEX-mScarlet-WPRE

   Plasmid

   #99280

   Purpose

   Can be used to generate AAV virus that will express mScarlet in the presence of Cre

   Depositor

   Rylan Larsen

   Insert

   mScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. hCCR5-mTFP1

   Plasmid

   #110193

   Purpose

   Encodes for human CCR5 coding sequence tagged with the mTFP1 FP

   Depositor

   Sergi Padilla Parra

   Article

   Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

   Insert

   hCCR5 (CCR5 Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-C1-TBP

   Plasmid

   #26674

   Depositor

   Sui Huang

   Article

   Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

   Insert

   TBP (TBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCINeo-myc-Itch (C822S)/2860

   Plasmid

   #11428

   Depositor

   Allan Weissman

   Article

   Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

   Insert

   Itch (Itch Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   cysteine at 822 mutated to serine

   Promoter

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.3_KLF4

   Plasmid

   #26815

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-KLF4-3'UTR (KLF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Starts from second ATG

   Promoter

   Available Since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pFR_CrPV_xb

    Plasmid

    #11509

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLNHA-C1-HsXRN1

    Plasmid

    #66596

    Purpose

    expresses lambdaN-HA-tagged XRN1 in mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    XRN1 (XRN1 Human)
    

    Use

    Tags

    lambdaN-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. phU1-PspCas13b-non-targeting (pEZ36)

    Plasmid

    #229053

    Purpose

    Cloning backbone for PspCas13b crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK5-FLAG-USP13

    Plasmid

    #61741

    Purpose

    express N-terminally FLAG tagged human USP13

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    USP13 (USP13 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti.CAG.H2B-cerFP.W.SINloxP

    Plasmid

    #51006

    Purpose

    Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-cerFP
    

    Use

    Lentiviral

    Tags

    histone 2B

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLminP_Luc2P_RE47

    Plasmid

    #90385

    Purpose

    IFNB1 - Interferon beta gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NFY

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AChE-pcDNA

    Plasmid

    #106442

    Purpose

    Mammalian expression of murine acetylcholinesterase

    Depositor

    Georg Weber

    Article

    Syed et al Clin Exp Metastasis. 2008;25(7):787-98.

    Insert

    acetylcholinesterase (ACHE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGRG25

    Plasmid

    #16665

    Depositor

    Nancy Craig

    Article

    McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

    Insert

    mTn7::MCS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-FnCas12a-TEV

    Plasmid

    #174658

    Purpose

    FnCas12a expression plasmid

    Depositor

    Huimin Zhao, Jin Wang

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-GFAP-hM3D(Gq)-mCherry

    Plasmid

    #50478

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter

    Depositor

    Bryan Roth

    Has Service

    AAV5

    Insert

    hM3D(Gq)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-Bxb1-His

    Plasmid

    #123132

    Purpose

    Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Bxb1 integrase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR223-PRKAG3

    Plasmid

    #23549

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

22. pLminP_Luc2P_RE60

    Plasmid

    #90405

    Purpose

    STAT5 - Stat5 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SIE

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMCSF-R-luc

    Plasmid

    #12420

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

    Insert

    M-CSF receptor promoter fragment (CSF1R Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

24. EGFP-Nsp7

    Plasmid

    #165112

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp7 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcdna3 flag - ULK1 wt

    Plasmid

    #27636

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse ULK1 (Ulk1 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Initial M from ULK1 removed.

    Promoter

    Available Since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPPED

    Plasmid

    #162468

    Purpose

    Destination Expression vector for dicot plant prime editing

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Inserts

    Cas9H840A
    attR gateway destination cassette
    

    Use

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine based sequence o…

    Promoter

    2x35S

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Rosa26 mT/mG

    Plasmid

    #17787

    Depositor

    Liqun Luo

    Article

    Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

    Insert

    Rosa26 mT/mG
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    membrane tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PE-Max-pet21A

    Plasmid

    #204471

    Purpose

    Expression plasmid for PE protein in bacteria.

    Depositor

    Scot Wolfe

    Article

    Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

    Insert

    Prime editor
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HA-HIF1alpha P402A/P564A-pBabe-puro

    Plasmid

    #19005

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 402 to Alanin…

    Promoter

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-ZDHHC21

    Plasmid

    #231669

    Purpose

    Expresses human ZDHHC21protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC21 (ZDHHC21 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge