We narrowed to 7,665 results for: RAP

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pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

Plasmid

#131684

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Inserts

EGFP-KASH
SpCas9 sgRNA vs mouse GRID1

Use

AAV

Tags

KASH

Promoter

EF1a and mU6-LSL (Cre dependent)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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AA320

Plasmid

#215952

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; sgCD47_v1 [EnAs]; DR_v1 [EnAs]; sgCD47_v2 [EnAs]}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA315

Plasmid

#215951

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA074

Plasmid

#215933

Purpose

Fragmid fragment: (guide cassette) Tet-regulatable guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

H1-2xTetO_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA435

Plasmid

#215962

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v0 [EnAs]; sgCD274_v1; DR_v1 [EnAs]; sgADGRE5_v3; DR_v2; sgCD4_v1; DR_v3; sgDPP4_v1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA021

Plasmid

#215929

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs];

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA083

Plasmid

#215934

Purpose

Fragmid fragment: (guide cassette) reverse orientation; guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP

Plasmid

#212795

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R/K63R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K48R/K63R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-Bat-SL-CoVZC45_PLP

Plasmid

#201939

Purpose

Expresses Bat-SL-CoVZC45 papain-like protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Bat-SL-CoVZC45 PLP

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-HCV_NS3-GS-NS4A

Plasmid

#203504

Purpose

Expresses HCV NS3-NS4A protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCV NS3-GS-NS4A

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-Bat-SL-CoVZC45_3CL

Plasmid

#201944

Purpose

Expresses Bat-SL-CoVZC45 3CL protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Bat-SL-CoVZC45 3CL protease

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-DUGV_OTU

Plasmid

#203463

Purpose

Expresses DUGV OTU domain of L protein from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

DUGV OTU domain of L protein (DUGVsLgp1 )

Expression

Yeast

Promoter

GAL10

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-3x-uC-HCoV-229E_3CL

Plasmid

#201937

Purpose

Expresses HCoV-229E 3CL protease from a GAL-3x promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-229E 3CL protease

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-HPeV_3C

Plasmid

#203465

Purpose

Expresses HPeV 3C protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HPeV 3C protease (polyprotein gene )

Expression

Yeast

Promoter

GAL10

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-AGPAT3-SVA

Plasmid

#202827

Purpose

pGL3 luciferase reporter with AGPAT3 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

intronic SVA located in AGPAT3 gene

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIH FRB-BFP

Plasmid

#202425

Purpose

TagBFP FKBP-rapamycin binding (FRB) dimerization domain

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

FKBP-rapamycin binding (FRB) dimerization domain

Use

Lentiviral

Tags

TagBFP

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-F1.GS.CAR-3G

Plasmid

#194462

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-F1.218.CAR-3G

Plasmid

#194460

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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Cox3-FusXTBE-Left TALE

Plasmid

#196504

Purpose

Expresses left-side TALE–DddAtox N term half cox3-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox3 left TALE-G1397 DddA-C-UGI-SV40 polyA

Expression

Bacterial

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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ND2-FusXTBE-Left TALE

Plasmid

#196498

Purpose

Expresses left-side TALE-DddAtox Cterm half ND2-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND2 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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