We narrowed to 11,247 results for: SHA

1. SaLgCG

   Plasmid

   #164563

   Purpose

   GFP

   Depositor

   Junwei Shi

   Article

   Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. IBMc180

   Plasmid

   #161942

   Purpose

   Carries Golden Gate substitution insert for acVHH-assisted bisection mapping

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 Ub-NLRP1 L1215

   Plasmid

   #166828

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221 Ub-NLRP1 L1218

   Plasmid

   #166829

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. dTAG NLRP1 P1214R

   Plasmid

   #166826

   Purpose

   dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P1214R

   Promoter

   hPGK

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. IBMc226

   Plasmid

   #161944

   Purpose

   Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDB.His.MBP.3C CARD8 CARD WT

   Plasmid

   #164022

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARD

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDB.His.MBP.3C CARD8 UPA-STOP

   Plasmid

   #164031

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CTMP

   Plasmid

   #129560

   Purpose

   cytoplasmic marker

   Depositor

   Inaki Ruiz-Trillo

   Article

   Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA-DAB1

    Plasmid

    #162691

    Purpose

    Expresses DabAB1 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB1 inorganic carbon transport complex
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SMT215

    Plasmid

    #134818

    Purpose

    SMT205 with DHFR-FLAG-tag, western blot

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SMT101

    Plasmid

    #134815

    Purpose

    Dual expression of DHFR and TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SMT201

    Plasmid

    #134816

    Purpose

    SMT101 with TET promoter for TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MYMH 351

    Plasmid

    #138058

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-10AG 11AC-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AAA GAG GAG GCA

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MYMH 336

    Plasmid

    #138057

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-1AG 2AC 11AC-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GCA GAG GAG ACA

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. MYMH 333

    Plasmid

    #138056

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-1AG 11AC-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GAA GAG GAG ACA

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MYMH 330

    Plasmid

    #138055

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb- 2AC 11AC-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ACA GAG GAG ACA

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MYMH 327

    Plasmid

    #138054

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-1AG 2AC-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GCA GAG GAG AAA

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJL1-neoR

    Plasmid

    #117048

    Purpose

    In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    neoR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pProEx-Htb-cytATL2-6Gly-sfGFP

    Plasmid

    #124065

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-sfGFP
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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